A Targetron-Recombinase System for Large-Scale Genome Engineering of Clostridia.

Cerisy T, Rostain W, Chhun A, Boutard M, Salanoubat M, Tolonen AC
mSphere. 2019 Dec 11;4(6). pii: 4/6/e00710-19. doi: 10.1128/mSphere.00710-19. PubMed Article

Plasmids from Article

ID	Plasmid	Purpose
135655	pQlox71F	Clostridial vector, encoding a targetron carrying a lox71 site in the forward (sense) orientation. Retarget intron to insert lox71 onto genome at desired site.
135656	pQlox71R	Clostridial vector, encoding a targetron carrying a lox71 site in the reverse (antisense) orientation. Retarget intron to insert lox71 onto genome at desired site.
135657	pQlox66R	Clostridial vector, encoding a targetron carrying a lox66 site in the reverse (antisense) orientation. Retarget intron to insert lox66 onto genome at desired site.
135658	pQlox66F	Clostridial vector, encoding a targetron carrying a lox66 site in the forward (sense) orientation. Retarget intron to insert lox66 onto genome at desired site.
135659	pQcre1	Clostridial vector, encoding the Cre protein, which mediates recombination between lox sites.
135660	pQadd1F	Clostridial vector, encoding a targetron carrying two lox511/71 and loxFAS/66 sites in tandem in the forward (sense) orientation. Retarget intron to insert double-lox site onto genome at desired site.
135661	pQadd1R	Clostridial vector, encoding a targetron carrying two lox511/71 and loxFAS/66 sites in tandem, in the reverse (antisense) orientation. Retarget intron to insert double-lox site at desired location.
135662	pQadd2	Clostridial vector, carrying lox511/66 and loxFAS/71 sites separated by restriction sites in order to add cargo. Use in combination with pQcre1 to insert cargo into lox sites, inserted with pQadd1F/R.