Search-and-replace genome editing without double-strand breaks or donor DNA.
Anzalone AV, Randolph PB, Davis JR, Sousa AA, Koblan LW, Levy JM, Chen PJ, Wilson C, Newby GA, Raguram A, Liu DR
Nature. 2019 Oct 21. pii: 10.1038/s41586-019-1711-4. doi: 10.1038/s41586-019-1711-4. PubMed Article
Anzalone AV, Randolph PB, Davis JR, Sousa AA, Koblan LW, Levy JM, Chen PJ, Wilson C, Newby GA, Raguram A, Liu DR
Nature. 2019 Oct 21. pii: 10.1038/s41586-019-1711-4. doi: 10.1038/s41586-019-1711-4. PubMed Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 132774 | pCMV-PE1 | Prime editing in mammalian cells |
| 132775 | pCMV-PE2 | Prime editing in mammalian cells. This plasmid is used by PE2, PE3, and PE3b |
| 132776 | pCMV-PE2-P2A-GFP | Prime editing in mammalian cells with co-translational GFP expression |
| 132777 | pU6-pegRNA-GG-acceptor | Prime editing in mammalian cells |
| 132778 | pU6-Sp-pegRNA-HEK3_CTT_ins | S. pyogenes pegRNA for CTT insertion at the HEK3 site of human cells using prime editing |
| 135955 | U6-DNMT1-hSynapsin-PE2-Nterminal-P2A-EGFP-KASH-lenti | Neuronal expression of N-terminal intein-split prime editor v2 (PE2) with EGFP-KASH marker and U6-mDNMT1 cassette. This plasmid is used by PE2, PE3, and PE3b |
| 135956 | hSynapsin-PE2-Cterminal-lenti | Neuronal expression of C-terminal intein-split prime editor v2 (PE2). This plasmid is used by PE2, PE3, and PE3b |
| 135957 | pU6-Sp-pegRNA-RNF2_+5GtoT | S. pyogenes pegRNA for +5 G to T edit at the RNF2 site of human cells using prime editing |
| 135958 | pU6-sp-sgRNA-RNF2_+41nick | S. pyogenes sgRNA for +41 nick in RNF2 |
| 135959 | pU6-sp-sgRNA-mDNMT1_nick-lenti | S. pyogenes nicking sgRNA for mouse DNMT1 |