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Unintended perturbation of protein function using GFP nanobodies in human cells.

Kuey C, Larocque G, Clarke NI, Royle SJ
J Cell Sci. 2019 Nov 6;132(21). pii: jcs.234955. doi: 10.1242/jcs.234955. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
128267pMito-mCherry-FRB_IRES_FKBP(I)-GBPenBicistronic vector to express mitochondrial targeted mCherry-FRB (MitoTrap) as well as 1x FKBP-GBPenhancer (GFP Nanobody with 1 FKBP domain).
128268pMito-mCherry-FRB_IRES_FKBP(III)-GBPenBicistronic vector to express mitochondrial targeted mCherry-FRB (MitoTrap) as well as 3x FKBP-GBPenhancer (GFP Nanobody with 3 FKBP domains).
128269pMito-GBPenExpression of mitochondrially targeted GBPen (GFP Nanobody)

Antibodies from Article