Maximizing binary interactome mapping with a minimal number of assays.
Choi SG, Olivet J, Cassonnet P, Vidalain PO, Luck K, Lambourne L, Spirohn K, Lemmens I, Dos Santos M, Demeret C, Jones L, Rangarajan S, Bian W, Coutant EP, Janin YL, van der Werf S, Trepte P, Wanker EE, De Las Rivas J, Tavernier J, Twizere JC, Hao T, Hill DE, Vidal M, Calderwood MA, Jacob Y
Nat Commun. 2019 Aug 29;10(1):3907. doi: 10.1038/s41467-019-11809-2.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 125547 | pDEST-N2H-N1 | Gateway compatible N2H assay destination vector with N-terminus tagging of nanoluc fragment1 |
| 125548 | pDEST-N2H-N2 | Gateway compatible N2H assay destination vector with N-terminus tagging of nanoluc fragment2 |
| 125549 | pDEST-N2H-C1 | Gateway compatible N2H assay destination vector with C-terminus tagging of nanoluc fragment1 |
| 125551 | pDEST-N2H-F1-empty vector | N2H assay vector with nanoluc fragment1 (empty control, no Gateway cloning site, expressing nanoluc fragment1) |
| 125552 | pDEST-N2H-F2-empty vector | N2H assay vector with nanoluc fragment2 (empty control, no Gateway cloning site, expressing nanoluc fragment2) |
| 125559 | pDEST-N2H-C2 | Gateway compatible N2H assay destination vector with C-terminus tagging of nanoluc fragment2 |