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An In Vitro Human Segmentation Clock Model Derived from Embryonic Stem Cells.
Chu LF, Mamott D, Ni Z, Bacher R, Liu C, Swanson S, Kendziorski C, Stewart R, Thomson JA
Cell Rep. 2019 Aug 27;28(9):2247-2255.e5. doi: 10.1016/j.celrep.2019.07.090.
PubMed Article

Plasmids from Article

ID Plasmid Purpose
130932HES7-NLuc-2A-tdTomatoDonor vector for tagging human HES7 C-terminus with NanoLuc and tdTomato
130933pCRII-Blunt-U6-HES7-STOP-gRNAgRNA for tag human HES7
130934pB-TAG-NICDPiggyBac vector for DOX-inducible human NICD-IRES-EGFP expression in mammalian cells
130935pB-TAG-HES7PiggyBac vector for DOX-inducible human HES7-IRES-EGFP expression in mammalian cells
130936pB-EF1a-NLuc-IRES-PuroPiggyBac vector for constitutive NanoLuc expression in mammalian cells

Antibodies from Article