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Systematic Immunotherapy Target Discovery Using Genome-Scale In Vivo CRISPR Screens in CD8 T Cells.

Dong MB, Wang G, Chow RD, Ye L, Zhu L, Dai X, Park JJ, Kim HR, Errami Y, Guzman CD, Zhou X, Chen KY, Renauer PA, Du Y, Shen J, Lam SZ, Zhou JJ, Lannin DR, Herbst RS, Chen S
Cell. 2019 Aug 22;178(5):1189-1204.e23. doi: 10.1016/j.cell.2019.07.044. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
192147pSC008_pLKO-U6-BsmBI-chRNA(+85)-EFS-Thy11T cell CRISPR lentiviral vector
192148pLY005SB_pAAV-U6sg(BbsI)-EFS-SB100XT cell CRISPR AAV vector
192149pRC54-DHX37-GFPexpress DHX37-GFP
192150pRC55-GFP-DHX37express GFP-DHX37

Antibodies from Article