De novo design of bioactive protein switches.
Langan RA, Boyken SE, Ng AH, Samson JA, Dods G, Westbrook AM, Nguyen TH, Lajoie MJ, Chen Z, Berger S, Mulligan VK, Dueber JE, Novak WRP, El-Samad H, Baker D
Nature. 2019 Jul 24. pii: 10.1038/s41586-019-1432-8. doi: 10.1038/s41586-019-1432-8.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 127200 | pAN1578 | Progesterone inducible expression of KeyA-BFP |
| 127201 | pAN1579 | Progesterone inducible expression of KeyC-BFP |
| 127202 | pAN1572 | Dual inducible expression of Venus-degronSwitchA and KeyA-BFP |
| 127203 | pAN1935 | Constitutive expression of Venus-degronSwitchA and mScarlet-degronSwitchC |
| 127204 | pAN1735 | Dual inducible expression of SynTF-mScarlet-degronSwitchA and KeyA-BFP-NLS |
| 127205 | pAN1646 | pTet expression of Venus, Dual inducible expression of dCas9-VP64-mScarlet-degronSwitchA and KeyA-BFP-NLS |
| 127206 | pAN1777 | Dual inducible expression of SynTF-mScarlet-nesSwitchA and KeyA-BFP-NLS |
| 127246 | pAN2981 | Asymmetric degronSwitchA_t8 fused to mCherry with KeyA-BFP |