Stability and sub-cellular localization of DNA polymerase beta is regulated by interactions with NQO1 and XRCC1 in response to oxidative stress.
Fang Q, Andrews J, Sharma N, Wilk A, Clark J, Slyskova J, Koczor CA, Lans H, Prakash A, Sobol RW
Nucleic Acids Res. 2019 Jul 9;47(12):6269-6286. doi: 10.1093/nar/gkz293.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 177131 | pGEX4T3-GST-PolB(WT) | Bacterial vector for expression of an N-terminal GST fusion of PolB(WT) with a TEV protease site located between the GST tag and PolB(WT) |
| 177132 | pGEX4T3-GST-PolB(K72A) | Bacterial vector for expression of an N-terminal GST fusion of PolB(K72A) with a TEV protease site located between the GST tag and PolB(K72A) |
| 177133 | pGEX4T3-GST-PolB(T304I) | Bacterial vector for expression of an N-terminal GST fusion of PolB(T304I) with a TEV protease site located between the GST tag and PolB(T304I) |
| 177134 | pGEX4T3-GST-PolB(K206A/K244A/T304I) | Bacterial vector for expression of an N-terminal GST fusion of PolB(K206A/K244A/T304I) with a TEV protease site located between the GST tag and PolB(K206A/K244A/T304I) |
| 177135 | pGEX4T3-GST-PolB(K206A/K244A) | Bacterial vector for expression of an N-terminal GST fusion of PolB(K206A/K244A) with a TEV protease site located between the GST tag and PolB(K206A/K244A) |
| 177136 | pGEX4T3-GST-EGFP | Bacterial vector for expression of an N-terminal GST fusion of EGFP with a TEV protease site located between the GST tag and EGFP |
| 177137 | pGEX4T3-GST-PolB(L301R/V303R/V306R) | Bacterial vector for expression of an N-terminal GST fusion of PolB(L301R/V303R/V306R) with a TEV protease site located between the GST tag and PolB(L301R/V303R/V306R) |
| 177138 | pGEX4T3-GST-PolB(K206A/K244A/L301R/V303R/V306R) | Bacterial vector for expression of an N-terminal GST fusion of PolB(K206A/K244A/L301R/V303R/V306R) with a TEV protease site located between the GST tag and PolB(K206A/K244A/L301R/V303R/V306R) |
| 177140 | pGEX4T3-GST-NQO1 | Bacterial vector for expression of an N-terminal GST fusion of NQO1 with a TEV protease site located between the GST tag and NQO1 |
| 177141 | pGEX4T3-GST-NQO1(Y128F) | Bacterial vector for expression of an N-terminal GST fusion of NQO1(Y128F) with a TEV protease site located between the GST tag and NQO1(Y128F) |
| 177142 | pGEX4T3-GST-Tev | Bacterial vector for expression of an N-terminal GST fusion of the Tev protease |
| 177143 | pLVX-Flag-PolB(T304I)-Puro | Lentiviral vector expressing Flag-PolB(T304I) and a puromycin resistance cassette |
| 177144 | pLVX-CMV-Flag-PolB(K244A/T304I)-Puro | Lentiviral vector expressing Flag-PolB(K244A/T304I) and a puromycin resistance cassette |
| 177145 | pLVX-CMV-Flag-PolB(K206A/K244A/T304I)-Puro | Lentiviral vector expressing Flag-PolB(K206A/K244A/T304I) and a puromycin resistance cassette |
| 177146 | pLentiGuide-PolB1-puro | Lentiviral vector expressing PolB-gRNA-1 and a puromycin resistance cassette |