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VEGAS as a Platform for Facile Directed Evolution in Mammalian Cells.

English JG, Olsen RHJ, Lansu K, Patel M, White K, Cockrell AS, Singh D, Strachan RT, Wacker D, Roth BL
Cell. 2019 Jul 2. pii: S0092-8674(19)30622-1. doi: 10.1016/j.cell.2019.05.051. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
127690pcDNA3.1 5HT2A_NB2_1Plasmid contains a positive allosteric modulating nanobody the binds to the cytoplasmic surface of 5HT2A.
127691pTREX GPR68_NB2_2Plasmid contains a nanobody that directly interacts with the cytoplasmic surface of GPR68.
127692pSRE SSGPlasmid expresses the Sindbis Structural Genome (SSG) via the serum response element (SRE) promoter. See Resource Information section for annotated plasmid sequence.
127693pSin CapsidPlasmid contains the Sindbis capsid gene and can be converted to mRNA for initiating Sindbis virus packaging. See Resource Information section for annotated plasmid sequence.
127694pSin HelperPlasmid contains the Sindbis glycoprotein genes and can be converted to mRNA for initiating Sindbis virus packaging. See Resource Information section for annotated plasmid sequence.
127695pTSin EGFPPlasmid contains the entire replication competent Sindbis genome with the structural genome components replaced by EGFP in an MCS locus. See Resource Information section for annotated plasmid sequence

Antibodies from Article