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A Two-Enzyme Adaptive Unit within Bacterial Folate Metabolism.
Schober AF, Mathis AD, Ingle C, Park JO, Chen L, Rabinowitz JD, Junier I, Rivoire O, Reynolds KA
Cell Rep. 2019 Jun 11;27(11):3359-3370.e7. doi: 10.1016/j.celrep.2019.05.030. PubMed Article

Plasmids from Article

ID Plasmid Purpose
91226pACYCDuet_WTDHFR_WTTSbacterial co-expression of folA (DHFR) and thyA(TS) with a barcode within the noncoding region between the genes
91227pACYCDuet_F31VDHFR_WTTSbacterial co-expression of folA (DHFR) and thyA(TS) with a barcode within the noncoding region between the genes
91228pACYCDuet_F31YG121VDHFR_R166QTSbacterial co-expression of folA (DHFR) and thyA(TS) with a barcode within the noncoding region between the genes
91229pACYCDuet_F31YG121VDHFR_WTTSbacterial co-expression of folA (DHFR) and thyA(TS) with a barcode within the noncoding region between the genes
91230pACYCDuet_F31YL54IDHFR_R166QTS bacterial co-expression of folA (DHFR) and thyA(TS) with a barcode within the noncoding region between the genes
91231pACYCDuet_F31YL54IDHFR_WTTSbacterial co-expression of folA (DHFR) and thyA(TS) with a barcode within the noncoding region between the genes
91232pACYCDuet_G121VDHFR_R166QTSbacterial co-expression of folA (DHFR) and thyA(TS) with a barcode within the noncoding region between the genes
91233pACYCDuet_G121VDHFR_WTTSbacterial co-expression of folA (DHFR) and thyA(TS) with a barcode within the noncoding region between the genes
91234pACYCDuet_L54IDHFR_R166QTSbacterial co-expression of folA (DHFR) and thyA(TS) with a barcode within the noncoding region between the genes
91235pACYCDuet_L54IDHFR_WTTSbacterial co-expression of folA (DHFR) and thyA(TS) with a barcode within the noncoding region between the genes
91236pACYCDuet_M42FDHFR_R166QTSbacterial co-expression of folA (DHFR) and thyA(TS) with a barcode within the noncoding region between the genes
91237pACYCDuet_M42FDHFR_WTTSbacterial co-expression of folA (DHFR) and thyA(TS) with a barcode within the noncoding region between the genes
91238pACYCDuet_M42FG121VDHFR_R166QTSbacterial co-expression of folA (DHFR) and thyA(TS) with a barcode within the noncoding region between the genes
91239pACYCDuet_M42FG121VDHFR_WTTSbacterial co-expression of folA (DHFR) and thyA(TS) with a barcode within the noncoding region between the genes
91240pACYCDuet_W22HDHFR_R166QTSbacterial co-expression of folA (DHFR) and thyA(TS) with a barcode within the noncoding region between the genes
91241pACYCDuet_W22HDHFR_WTTSbacterial co-expression of folA (DHFR) and thyA(TS) with a barcode within the noncoding region between the genes
91242pACYCDuet_WTEcoliDHFR_R166QTSbacterial co-expression of folA (DHFR) and thyA(TS) with a barcode within the noncoding region between the genes
91243pACYCDuet_D27NDHFR_R166QTSbacterial co-expression of folA (DHFR) and thyA(TS) with a barcode within the noncoding region between the genes
91244pACYCDuet_D27NDHFR_WTTSbacterial co-expression of folA (DHFR) and thyA(TS) with a barcode within the noncoding region between the genes
91245pACYCDuet_F31VDHFR_R166QTSbacterial co-expression of folA (DHFR) and thyA(TS) with a barcode within the noncoding region between the genes

Antibodies from Article