RNA-guided DNA insertion with CRISPR-associated transposases.
Strecker J, Ladha A, Gardner Z, Schmid-Burgk JL, Makarova KS, Koonin EV, Zhang F
Science. 2019 Jul 5;365(6448):48-53. doi: 10.1126/science.aax9181. Epub 2019 Jun 6.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 127921 | pHelper_ShCAST_sgRNA | Expresses ShCAST and an empty sgRNA scaffold. New targets can be added using Golden Gate assembly (LguI sites). |
| 127922 | pHelper_ShCAST | Expresses ShCAST and a crRNA targeting PSP1 |
| 127923 | pHelper_AcCAST | Expresses AcCAST and a crRNA targeting PSP1 |
| 127924 | pDonor_ShCAST_kanR | Kanamycin resistance donor for ShCAST |
| 127925 | pDonor_AcCAST_kanR | Kanamycin resistance donor for AcCAST |
| 127926 | pTarget_CAST | Target plasmid containing PSP1 |
| 135524 | pXT128_TwinStrep-SUMO-ShCas12k | Protein expression construct for ShCast12k |
| 135525 | pXT129_TwinStrep-SUMO-ShTnsB | Protein expression construct for ShTnsB |
| 135526 | pXT130_TwinStrep-SUMO-ShTnsC | Protein expression construct for ShTnsC |
| 135527 | pXT131_TwinStrep-SUMO-ShTniQ | Protein expression construct for ShTniQ |