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Ribosome queuing enables non-AUG translation to be resistant to multiple protein synthesis inhibitors.
Kearse MG, Goldman DH, Choi J, Nwaezeapu C, Liang D, Green KM, Goldstrohm AC, Todd PK, Green R, Wilusz JE
Genes Dev. 2019 Jun 6. pii: gad.324715.119. doi: 10.1101/gad.324715.119. PubMed Article

Plasmids from Article

ID Plasmid Purpose
127299pcDNA3.1(+)/AUG-nLuc-3XFLAGExpress 3xFLAG tagged nanoLuciferase (nLuc) from AUG start codon
127300pcDNA3.1(+)/CUG-nLuc-3XFLAGExpress 3xFLAG tagged nanoLuciferase (nLuc) from CUG start codon
127301pcDNA3.1(+)/GUG-nLuc-3XFLAGExpress 3xFLAG tagged nanoLuciferase (nLuc) from GUG start codon
127302pcDNA3.1(+)/UUG-nLuc-3XFLAGExpress 3xFLAG tagged nanoLuciferase (nLuc) from UUG start codon
127303pcDNA3.1(+)/ACG-nLuc-3XFLAGExpress 3xFLAG tagged nanoLuciferase (nLuc) from ACG start codon
127304pcDNA3.1(+)/AGG-nLuc-3XFLAGExpress 3xFLAG tagged nanoLuciferase (nLuc) from AGG start codon
127305pcDNA3.1(+)/AUU-nLuc-3XFLAGExpress 3xFLAG tagged nanoLuciferase (nLuc) from AUU start codon
127306pcDNA3.1(+)/AAA-nLuc-3XFLAGExpress 3xFLAG tagged nanoLuciferase (nLuc) from AAA start codon (Negative control plasmid)
127307pcDNA3.1(+)/GGG-nLuc-3XFLAGExpress 3xFLAG tagged nanoLuciferase (nLuc) from GGG start codon (Negative control plasmid)
127308pcDNA3.1(+)/AUG-stop-nLuc-3XFLAGExpress 3xFLAG tagged nanoLuciferase (nLuc) from AUG start codon, but translation terminated immediately by stop codon
127309pcDNA3.1(+)/CUG-stop-nLuc-3XFLAGExpress 3xFLAG tagged nanoLuciferase (nLuc) from CUG start codon, but translation terminated immediately by stop codon
127310pcDNA3.1(+)/AAA-stop-nLuc-3XFLAGExpress 3xFLAG tagged nanoLuciferase (nLuc) from negative control AAA start codon, but translation terminated immediately by stop codon
127311pcDNA3.1(+)/GGG-stop-nLuc-3XFLAGExpress 3xFLAG tagged nanoLuciferase (nLuc) from negative control GGG start codon, but translation terminated immediately by stop codon
127312pcDNA3.1(+)/AUG-nLuc-3XFLAG-PESTExpress 3xFLAG tagged destabilized nanoLuciferase (nLuc) protein from AUG start codon
127313pcDNA3.1(+)/CUG-nLuc-3XFLAG-PESTExpress 3xFLAG tagged destabilized nanoLuciferase (nLuc) protein from CUG start codon
127314pcDNA3.1(+)/GUG-nLuc-3XFLAG-PESTExpress 3xFLAG tagged destabilized nanoLuciferase (nLuc) protein from GUG start codon
127315pcDNA3.1(+)/AUG-nLuc-3XFLAG-CL1/PESTExpress 3xFLAG tagged highly destabilized nanoLuciferase (nLuc) protein from AUG start codon
127316pcDNA3.1(+)/CUG-nLuc-3XFLAG-CL1/PESTExpress 3xFLAG tagged highly destabilized nanoLuciferase (nLuc) protein from CUG start codon
127317pcDNA3.1(+)/GUG-nLuc-3XFLAG-CL1/PESTExpress 3xFLAG tagged highly destabilized nanoLuciferase (nLuc) protein from GUG start codon
127318pcDNA3.1(+)/ACG-nLuc-3XFLAG-CL1/PESTExpress 3xFLAG tagged highly destabilized nanoLuciferase (nLuc) protein from ACG start codon
127319pcDNA3.1(+)/AUU-nLuc-3XFLAG-CL1/PESTExpress 3xFLAG tagged highly destabilized nanoLuciferase (nLuc) protein from AUU start codon
127320pcDNA3.1(+)/AAA-nLuc-3XFLAG-CL1/PESTExpress 3xFLAG tagged highly destabilized nanoLuciferase (nLuc) protein from AAA start codon (Negative control plasmid)
127321pcDNA3.1(+)/GGG-nLuc-3XFLAG-CL1/PESTExpress 3xFLAG tagged highly destabilized nanoLuciferase (nLuc) protein from GGG start codon (Negative control plasmid)
127322pcDNA3.1(+)/HP + AUG-nLuc-3XFLAGExpress 3xFLAG tagged nanoLuciferase (nLuc) from AUG start codon from mRNA with hairpin in 5' UTR
127323pcDNA3.1(+)/HP + GUG-nLuc-3XFLAGExpress 3xFLAG tagged nanoLuciferase (nLuc) from GUG start codon from mRNA with hairpin in 5' UTR
127324pcDNA3.1(+)/(CAA)26 5′ leader AUG-nLuc-3XFLAGExpress 3xFLAG tagged nanoLuciferase (nLuc) from AUG start codon from mRNA with (CAA)26 repeats in 5' UTR
127325pcDNA3.1(+)/(CAA)26 5′ leader GUG-nLuc-3XFLAGExpress 3xFLAG tagged nanoLuciferase (nLuc) from GUG start codon from mRNA with (CAA)26 repeats in 5' UTR
127326pcDNA3.1(+)/(CAA)12 + (AGAGAG)7 5′ leader AUG-nLuc-3XFLAGExpress 3xFLAG tagged nanoLuciferase (nLuc) from AUG start codon from mRNA with (AGAGAG)7 repeats in 5' UTR
127327pcDNA3.1(+)/(CAA)12 + (AGAGAG)7 5′ leader GUG-nLuc-3XFLAGExpress 3xFLAG tagged nanoLuciferase (nLuc) from GUG start codon from mRNA with (AGAGAG)7 repeats in 5' UTR
127328pcDNA3.1(+)/BAG1 5′ UTR CUG-nLuc-3xFLAGExpress 3xFLAG tagged nanoLuciferase (nLuc) from CUG start codon from mRNA with BAG1 5' UTR
127329pcDNA3.1(+)/BAG1 5′ UTR AUG-nLuc-3xFLAGExpress 3xFLAG tagged nanoLuciferase (nLuc) from AUG start codon from mRNA with BAG1 5' UTR
127330pcDNA3.1(+)/GUG-DAP5-3XFLAGExpress 3xFLAG tagged DAP5 protein from GUG start codon
127331pcDNA3.1(+)/AUG-DAP5-3XFLAGExpress 3xFLAG tagged DAP5 protein from AUG start codon
127332pcDNA3.1D/mEGFP + CrPV IGR IRES-nLuc-3XFLAG-CL1PESTExpress 3xFLAG tagged highly destabilized nanoLuciferase (nLuc) protein from CrPV IRES
127333pGL4.13/AUG-FFLuc-3XFLAGExpress 3xFLAG tagged firefly luciferase (FFLuc) from AUG start codon
127334pGL4.13/GUG-FFLuc-3XFLAGExpress 3xFLAG tagged firefly luciferase (FFLuc) from GUG start codon
127335pGL4.13/GUG, AAA(30)-FFLuc-3XFLAGExpress 3xFLAG tagged firefly luciferase (FFLuc) from GUG start codon (AUG at codon 30 mutated to AAA)
127336pGL4.13/AUG(30)-FFLuc-3XFLAGExpress truncated 3xFLAG tagged firefly luciferase (FFLuc) from AUG at codon 30
127337pGL4.13/AUG(59)-FFLuc-3XFLAGExpress truncated 3xFLAG tagged firefly luciferase (FFLuc) from AUG at codon 59

Antibodies from Article