Escherichia coli vectors having stringently repressible replication origins allow a streamlining of Crispr/Cas9 gene editing.

Srinivas S, Hu Z, Cronan JE
Plasmid. 2019 May;103:53-62. doi: 10.1016/j.plasmid.2019.04.004. Epub 2019 Apr 29. PubMed Article

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