Renal medullary carcinomas depend upon SMARCB1 loss and are sensitive to proteasome inhibition.
Hong AL, Tseng YY, Wala JA, Kim WJ, Kynnap BD, Doshi MB, Kugener G, Sandoval GJ, Howard TP, Li J, Yang X, Tillgren M, Ghandi M, Sayeed A, Deasy R, Ward A, McSteen B, Labella KM, Keskula P, Tracy A, Connor C, Clinton CM, Church AJ, Crompton BD, Janeway KA, Van Hare B, Sandak D, Gjoerup O, Bandopadhayay P, Clemons PA, Schreiber SL, Root DE, Gokhale PC, Chi SN, Mullen EA, Roberts CW, Kadoch C, Beroukhim R, Ligon KL, Boehm JS, Hahn WC
Elife. 2019 Mar 12;8. pii: 44161. doi: 10.7554/eLife.44161. PubMed Article
Hong AL, Tseng YY, Wala JA, Kim WJ, Kynnap BD, Doshi MB, Kugener G, Sandoval GJ, Howard TP, Li J, Yang X, Tillgren M, Ghandi M, Sayeed A, Deasy R, Ward A, McSteen B, Labella KM, Keskula P, Tracy A, Connor C, Clinton CM, Church AJ, Crompton BD, Janeway KA, Van Hare B, Sandak D, Gjoerup O, Bandopadhayay P, Clemons PA, Schreiber SL, Root DE, Gokhale PC, Chi SN, Mullen EA, Roberts CW, Kadoch C, Beroukhim R, Ligon KL, Boehm JS, Hahn WC
Elife. 2019 Mar 12;8. pii: 44161. doi: 10.7554/eLife.44161. PubMed Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 111181 | pDONR SMARCB1 var 2 | SMARCB1 variant 2 in pDONR223 backbone |
| 111182 | pLXI_TRC401 SMARCB1 var 2 | Inducible vector (untagged) with SMARCB1 variant 2 |
| 111183 | pLXI_TRC401 LacZ | Control inducible vector with LacZ (V5 tagged) |
| 111184 | pLXI_TRC403 LacZ | Control inducible vector with LacZ (V5 tagged) |
| 111185 | pLXI_TRC403 SMARCB1 var 2 | Inducible vector (V5 tagged) with SMARCB1 variant 2 |
| 117068 | pXPR_BRD003 UBE2C guide 1 | single guide RNA targeting UBE2C; guide 1 |
| 117071 | pXPR_BRD003 UBE2C guide 2 | single guide RNA targeting UBE2C; guide 2 |
| 117072 | pXPR_BRD003 Luciferase | single guide RNA targeting Luciferase |
| 117073 | pXPR_BRD003 PSMB5 guide 1 | single guide RNA targeting PSMB5; guide 1 |
| 117074 | pXPR_BRD003 PSMB5 guide 2 | single guide RNA targeting PSMB5; guide 2 |