Neuron-Specific Genome Modification in the Adult Rat Brain Using CRISPR-Cas9 Transgenic Rats.
Back S, Necarsulmer J, Whitaker LR, Coke LM, Koivula P, Heathward EJ, Fortuno LV, Zhang Y, Yeh CG, Baldwin HA, Spencer MD, Mejias-Aponte CA, Pickel J, Hoffman AF, Spivak CE, Lupica CR, Underhill SM, Amara SG, Domanskyi A, Anttila JE, Airavaara M, Hope BT, Hamra FK, Richie CT, Harvey BK
Neuron. 2019 Feb 8. pii: S0896-6273(19)30062-5. doi: 10.1016/j.neuron.2019.01.035. PubMed Article
Back S, Necarsulmer J, Whitaker LR, Coke LM, Koivula P, Heathward EJ, Fortuno LV, Zhang Y, Yeh CG, Baldwin HA, Spencer MD, Mejias-Aponte CA, Pickel J, Hoffman AF, Spivak CE, Lupica CR, Underhill SM, Amara SG, Domanskyi A, Anttila JE, Airavaara M, Hope BT, Hamra FK, Richie CT, Harvey BK
Neuron. 2019 Feb 8. pii: S0896-6273(19)30062-5. doi: 10.1016/j.neuron.2019.01.035. PubMed Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 112677 | pOTTC1032 - pAAV EF1a Nuc-flox(mCherry)-EGFP | An AAV vector that expresses a Cre-dependent nuclear-localized Red to Green Fluorescent protein |
| 112683 | pOTTC1442 - pAAV CMV-IE Nuc-iRFP-2A-iCre | An AAV vector that expresses a nuclear-localized iRFP713 reporter and improved Cre recombinase |
| 112684 | pOTTC1440 - pAAV CMV-IE Nuc-iRFP-2A-Flpo | An AAV vector that expresses a nuclear-localized iRFP713 reporter and optimized Flp recombinase |
| 112719 | pOTTC1124 - pAAV MeCP2 SpCas9(D10A) | An AAV vector that expresses SpCas9 nickase under a neuronal cell promoter |
| 113155 | pOTTC1088 - pAAV TH gRNA A+B pair EF1a EGFP | An AAV vector that expresses guide RNAs targeting rat TH and expresses EGFP reporter |
| 113156 | pOTTC1270 - pAAV Rosa26 gRNA A+B EF1a EGFP | An AAV vector that expresses guide RNAs targeting rat Rosa26 and expresses EGFP reporter |
| 113157 | pOTTC1388 - pAAV MANF gRNA A+B EF1a EGFP | An AAV vector that expresses guide RNAs targeting rat MANF and expresses EGFP reporter |
| 113158 | pOTTC1179 - pAAV (flox-stop) TH gRNA A EF1a eGFP | An AAV vector that expresses a Cre-dependent guide RNA targeting rat TH and expresses EGFP reporter |
| 113159 | pOTTC1079 - pAAV TH gRNA A EF1a EGFP | An AAV vector that expresses guide RNA targeting rat TH and expresses EGFP reporter |
| 113160 | pOTTC1181 - pmU6(loxP-STOP-loxP) BbsI gRNA | A plasmid for cloning Cre-dependent guide RNAs using a modified mouse U6 promoter containing loxP |
| 113161 | pOTTC763 - pX458 with rat Rosa26 gRNA A | A plasmid that expresses a guide RNA targeting rat Rosa26 as well as FLAG-tagged SpCas9 and GFP |
| 113162 | pOTTC1076 - prRosa26v1 LSL FLAG-SpCas9n NeoR | A donor plasmid with homologous arms matching rat Rosa26 and expressing a Cre-dependent FLAG-tagged SpCas9n and a NeoR selectable marker |
| 113163 | pOTTC1080 - prRosa26v2 LSL FLAG-SpCas9 | A donor plasmid with homologous arms matching rat Rosa26 and expressing a Cre-dependent FLAG-tagged SpCas9 |