In vivo characterisation of fluorescent proteins in budding yeast.
Botman D, de Groot DH, Schmidt P, Goedhart J, Teusink B
Sci Rep. 2019 Feb 19;9(1):2234. doi: 10.1038/s41598-019-38913-z. PubMed Article
Botman D, de Groot DH, Schmidt P, Goedhart J, Teusink B
Sci Rep. 2019 Feb 19;9(1):2234. doi: 10.1038/s41598-019-38913-z. PubMed Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 118421 | pDRF1-GW eCFP-T2A-mCherry | Produces equimolar expression of eCFP and mCherry |
| 118422 | pDRF1-GW eGFP-T2A-mCherry | Produces equimolar expression of eGFP and mCherry |
| 118423 | pDRF1-GW mCherry-T2A-mTurquoise2 | Produces equimolar expression of mCherry and mTq2 |
| 118424 | pDRF1-GW Citrine-T2A-mTurquoise2 | Produces equimolar expression of Citrine and mTq2 |
| 118425 | pDRF1-GW Clover-T2A-mTurquoise2 | Produces equimolar expression of Clover and mTq2 |
| 118426 | pDRF1-GW mKate2-T2A-mTurquoise2 | Produces equimolar expression of mKate2 and mTq2 |
| 118427 | pDRF1-GW mKo2-T2A-mTurquoise2 | Produces equimolar expression of mKo2 and mTq2 |
| 118428 | pDRF1-GW mkok-T2A-mTurquoise2 | Produces equimolar expression of mKokappa and mTq2 |
| 118429 | pDRF1-GW mRuby2-T2A-mTurquoise2 | Produces equimolar expression of mRuby2 and mTq2 |
| 118430 | pDRF1-GW mScarletI-T2A-mTurquoise2 | Produces equimolar expression of mScarletI and mTq2 |
| 118431 | pDRF1-GW mScarlet-T2A-mTurquoise2 | Produces equimolar expression of mScarlet and mTq2 |
| 118432 | pDRF1-GW mTFP-T2A-mCherry | Produces equimolar expression of mTFP and mCherry |
| 118433 | pDRF1-GW mTurquoise2-T2A-mcherry | Produces equimolar expression of mTq2 and mCherry |
| 118434 | pDRF1-GW mVenus-T2A-mTurquoise2 | Produces equimolar expression of mVenus and mTq2 |
| 118435 | pDRF1-GW sGFP2-T2A-mCherry | Produces equimolar expression of sGFP2 and mCherry |
| 118436 | pDRF1-GW sYFP2-T2A-mTurquoise2 | Produces equimolar expression of sYFP2 and mTq2 |
| 118437 | pDRF1-GW tagRFP-T2A-mTurquoise2 | Produces equimolar expression of tagRFP and mTq2 |
| 118438 | pDRF1-GW tagRFPT-T2A-mTurquoise2 | Produces equimolar expression of tagRFPT and mTq2 |
| 118439 | pDRF1-GW tdTomato-T2A-mTurquoise2 | Produces equimolar expression of tdTomato and mTq2 |
| 118440 | pDRF1-GW yeVenus-T2A-mTurquoise2 | Produces equimolar expression of yeVenus and mTq2 |
| 118441 | pDRF1-GW ymScarletI-T2A-mTurquoise2 | Produces equimolar expression of yeast codon-optimized mScarletI and mTq2 |
| 118442 | pDRF1-GW ymTurquoise2-T2A-mCherry | Produces equimolar expression of yeast codon-optimized mTq2 and mCherry |
| 118443 | pDRF1-GW ymVenus-T2A-mTurquoise2 | Produces equimolar expression of yeast codon-optimized mVenus and mTq2 |
| 118444 | pDRF1-GW ymYPET-T2A-mTurquoise2 | Produces equimolar expression of ymYPET and mTq2 |
| 118445 | pDRF1-GW yoeCFP-T2A-mCherry | Produces equimolar expression of yoeCFP and mCherry |
| 118446 | pDRF1-GW yoeGFP-T2A-mCherry | Produces equimolar expression of yeast codon-optimized eGFP and mCherry |
| 118447 | pDRF1-GW yomCherry-T2A-mTurquoise2 | Produces equimolar expression of yeast codon-optimized mCherry and mTq2 |
| 118448 | pDRF1-GW yosfGFP-T2A-mCherry | Produces equimolar expression of yeast codon-optimized sfGFP and mCherry |
| 118449 | pDRF1-GW yotagRFPT-T2A-mTurquoise2 | Produces equimolar expression of yeast codon-optimized tagRFPT and mTq2 |
| 118450 | pDRF1-GW YPET-T2A-mTurquoise2 | Produces equimolar expression of YPET and mTq2 |
| 118451 | pDRF1-GW ytdtomato-T2A-mTurquoise2 | Produces equimolar expression of yeast codon-optimized tdTomato and mTq2 |
| 118452 | pDRF1-GW ymScarletI | Overexpression of yeast codon-optimized mScarletI |
| 118453 | pDRF1-GW ymTurquoise2 | Overexpression of yeast codon-optimized mTq2 |
| 118454 | pDRF1-GW ymVenus | Overexpression of yeast codon-optimized mVenus |
| 118455 | pDRF1-GW ymYPET | Overexpression of ymYPET |
| 118456 | pDRF1-GW ytdTomato | Overexpression of yeast codon-optimized tdTomato |
| 118457 | pFA6a-ymScarletI-CaURA3 | Yeast tagging vector to create a gene fusion with yeast optimized mScarletI with CaUra3 selection |
| 118458 | pFA6a-ymTurquoise2-CaURA3 | Yeast tagging vector to create a gene fusion with yeast optimized mTq2 with CaUra3 selection |
| 118459 | pFA6a-ymVenus-CaURA3 | Yeast tagging vector to create a gene fusion with yeast optimized mVenus with CaUra3 selection |
| 118460 | pFA6a-ymYPET-CaURA3 | Yeast tagging vector to create a gene fusion with ymYPET with CaUra3 selection |
| 118461 | pFA6a-ytdTomato-CaURA3 | Yeast tagging vector to create a gene fusion with yeast optimized tdTomato with CaUra3 selection |
| 125698 | pDRF1-GW mNeongreen-T2A-mCherry | Produces equimolar expression of mNeongreen and mCherry |
| 125700 | pDRF1-GW ymNeongreen-T2A-mCherry | Produces equimolar expression of yeast codon optimized mNeongreen and mCherry |
| 125701 | pDRF1-GW ymNeongreen-T2A-mTurquoise2 | Produces equimolar expression of yeast codon optimized mNeongreen and mTurquoise2 |
| 125703 | pFA6a-ymNeongreen-CaURA3 | Yeast tagging vector to create a gene fusion with yeast optimized mNeongreen with CaUra3 selection |
| 125704 | pFA6a-link-ymNeongreen-SpHis5 | Yeast tagging vector to create a gene fusion with yeast optimized mNeongreen with SpHIS5 selection |
| 168054 | pFA6a-link-ytdTomato-URA3 | Replaces Addgene plasmid 118461. Yeast tagging vector to create a gene fusion with yeast optimized tdTomato with CaUra3 selection; Contains the linker region at the 5’ end of the FP |
| 168055 | pFA6a-link-ymScarletI-URA3 | Replaces Addgene plasmid 118457. Yeast tagging vector to create a gene fusion with yeast optimized mScarletI with CaUra3 selection; Contains the linker region at the 5’ end of the FP |
| 168056 | pFA6a-link-ymYPET-URA3 | Replaces Addgene plasmid 118460. Yeast tagging vector to create a gene fusion with yeast optimized mYPET with CaUra3 selection; Contains the linker region at the 5’ end of the FP |
| 168057 | pFA6a-link-ymTq2-URA3 | Replaces Addgene Plasmid 118458. Yeast tagging vector to create a gene fusion with yeast optimized mTurquoise2 with CaUra3 selection; Contains the linker region at the 5’ end of the FP |
| 168058 | pFA6a-link-ymVenus-URA3 | Replaces Addgene plasmid 118459. Yeast tagging vector to create a gene fusion with yeast optimized mVenus with CaUra3 selection; Contains the linker region at the 5’ end of the FP |
| 168059 | pFA6a-link-ymNeongreen-URA3 | Replaces Addgene Plasmid 125703. Yeast tagging vector to create a gene fusion with yeast optimized mNeongreen with CaUra3 selection; Contains the linker region at the 5’ end of the FP |
| 168078 | cyterm-mTq2 pDRF1-GW | Expresses Cyterm-mTq2 to check for monomericity. |
| 168079 | cyterm-ymVenus pDRF1-GW | Expresses Cyterm-mVenus to check for monomericity. |
| 168080 | cyterm-yeVenus pDRF1-GW | Expresses Cyterm-yeVenus to check for monomericity. |
| 168081 | cyterm-tagRFPT pDRF1-GW | Expresses Cyterm-tagRFPT to check for monomericity. |
| 168082 | cyterm-ymScarletI pDRF1-GW | Expresses Cyterm-ymScarletI to check for monomericity. |
| 168083 | cyterm-ymtq2 pDRF1-GW | Expresses Cyterm-ymTq2 to check for monomericity. |