Short-Homology-Mediated CRISPR/Cas9-Based Method for Genome Editing in Fission Yeast.
Hayashi A, Tanaka K
G3 (Bethesda). 2019 Feb 12. pii: g3.118.200976. doi: 10.1534/g3.118.200976. PubMed Article
Hayashi A, Tanaka K
G3 (Bethesda). 2019 Feb 12. pii: g3.118.200976. doi: 10.1534/g3.118.200976. PubMed Article
Plasmids from Article
ID | Plasmid | Purpose | |
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121144 | pAH233 | Expression of gRNA with BbsI placeholder for target cloning |
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121145 | pAH235 | Cas9 expression controlled by the nmt41 promoter |
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121146 | pAH237 | Expression of both nmt41p-cas9 and rrk1p-gRNA (LEU2 marker) for CRISPR genome editing in fission yeast |
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121147 | pAH243 | Expression of both nmt41p-cas9 and rrk1p-gRNA (ura4 marker) for CRISPR genome editing in fission yeast |
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