Structural architecture of a dimeric class C GPCR based on co-trafficking of sweet taste receptor subunits.
Park J, Selvam B, Sanematsu K, Shigemura N, Shukla D, Procko E
J Biol Chem. 2019 Feb 5. pii: RA118.006173. doi: 10.1074/jbc.RA118.006173.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 113944 | pcDNA3.1(+)-HA-Flag-natT1R2 | mammalian expression plasmid for FLAG-tagged human T1R2 with signal peptide of influenza hemagglutinin |
| 113945 | pCEP4-HA-Flag-natT1R2 | mammalian expression plasmid for FLAG-tagged human T1R2 with signal peptide of influenza hemagglutinin |
| 113946 | pcDNA3.1(+)-HLA-cmyc-natT1R2 | mammalian expression plasmid for c-myc-tagged human T1R2 with signal peptide of HLA class I histocompatibility antigen A-2 alpha chain |
| 113947 | pcDNA3.1(+)-HLA-Flag-natT1R2 | mammalian expression plasmid for FLAG-tagged human T1R2 with signal peptide of HLA class I histocompatibility antigen A-2 alpha chain |
| 113948 | pCEP4-HLA-cmyc-optiT1R3 a21-852 | mammalian expression plasmid for c-myc-tagged human codon-optimized human T1R3 with signal peptide of HLA class I histocompatibility antigen A-2 alpha chain |
| 113949 | pcDNA3.1(+)-HLA-cmyc-optiT1R3 a21-852 | mammalian expression plasmid for c-myc-tagged human codon-optimized human T1R3 with signal peptide of HLA class I histocompatibility antigen A-2 alpha chain |
| 113950 | pcDNA3.1(+)-HLA-Flag-natT1R3 | mammalian expression plasmid for FLAG-tagged human T1R3 with signal peptide of HLA class I histocompatibility antigen A-2 alpha chain |
| 113951 | pCEP4-HA-Flag-natT1R2 a22-end_S212N | mammalian expression plasmid for FLAG-tagged human T1R2 with signal peptide of influenza hemagglutinin; expression-enhanced variant |
| 113952 | pCEP4-HA-Flag-natT1R2 a22-end_I132S-S212N | mammalian expression plasmid for FLAG-tagged human T1R2 with signal peptide of influenza hemagglutinin; expression-enhanced variant |
| 113953 | pCEP4-HA-Flag-natT1R2 a22-end_I132S-S212N-D231G | mammalian expression plasmid for FLAG-tagged human T1R2 with signal peptide of influenza hemagglutinin; expression-enhanced variant |
| 113954 | pCEP4-HA-Flag-natT1R2 ECD | mammalian expression plasmid for FLAG-tagged human T1R2 extracellular domain with signal peptide of influenza hemagglutinin |
| 113955 | pCEP4-HA-Flag-natT1R2 FS1 | mammalian expression plasmid for FLAG-tagged human T1R2 with signal peptide of influenza hemagglutinin; expression-enhanced variant |
| 113956 | pCEP4-HA-Flag-natT1R2 FS10 | mammalian expression plasmid for FLAG-tagged human T1R2 with signal peptide of influenza hemagglutinin; expression-enhanced variant |
| 113957 | pCEP4-HA-Flag-natT1R2 ECD-MHC | mammalian expression plasmid for FLAG-tagged human T1R2 ECD with signal peptide of influenza hemagglutinin fused to a canonical transmembrane domain from MHC class I |
| 113958 | pCEP4-HA-Flag-natT1R2 RR837-8GG | mammalian expression plasmid for FLAG-tagged human T1R2 with signal peptide of influenza hemagglutinin; expression-enhanced variant |
| 113959 | pCEP4-HA-Flag-natT1R2 d835 | mammalian expression plasmid for FLAG-tagged human T1R2 with signal peptide of influenza hemagglutinin; expression-enhanced variant |
| 113960 | pCEP4-HLA-c-myc-optiT1R3 ECD | mammalian expression plasmid for c-myc-tagged human codon-optimized human T1R3 extracellular domain with signal peptide of HLA class I histocompatibility antigen A-2 alpha chain |
| 113961 | pCEP4-HLA-c-myc-optiT1R3 ECD-MHC | mammalian expression plasmid for c-myc-tagged human codon-optimized human T1R3 extracellular domain with signal peptide of HLA class I histocompatibility antigen A-2 alpha chain fused to a canonical |
| 113962 | pcDNA3-HLA-cMyc-EcopT1R1 | mammalian expression plasmid for c-myc-tagged E. coli codon-optimized human T1R1 with signal peptide of HLA class I histocompatibility antigen A-2 alpha chain |