T cell antigen discovery via signaling and antigen-presenting bifunctional receptors.
Joglekar AV, Leonard MT, Jeppson JD, Swift M, Li G, Wong S, Peng S, Zaretsky JM, Heath JR, Ribas A, Bethune MT, Baltimore D
Nat Methods. 2019 Feb;16(2):191-198. doi: 10.1038/s41592-018-0304-8. Epub 2019 Jan 28.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 119050 | pCCLc-MND-A0201-SABR-Backbone | 3rd gen transfer vector. HLA-A0201 linked to b2-microglobulin and CD28-CD3z signaling domain. Contains a stuffer fragment flanked by BsmBI sites at the epitope cloning site. |
| 119051 | pCCLc-MND-B2705-SABR-Backbone | 3rd gen transfer vector. HLA-B2705 linked to b2-microglobulin and CD28-CD3z signaling domain. Contains a stuffer fragment flanked by BsmBI sites at the epitope cloning site. |
| 119052 | pCCLc-MND-A0201-Mart1-SABR | 3rd gen transfer vector. HLA-A0201 linked to b2-microglobulin and CD28-CD3z signaling domain. Contains the Mart1 epitope "ELAGIGILTV" cloned via BsmBI |
| 119053 | pCCLc-MND-B2705-KK10-SABR | 3rd gen transfer vector. HLA-B2705 linked to b2-microglobulin and CD28-CD3z signaling domain. Contains the HIV(gag) KK10 epitope "KRWIILGLNK" cloned via BsmBI |