Enabling genetic analysis of diverse bacteria with Mobile-CRISPRi.
Peters JM, Koo BM, Patino R, Heussler GE, Hearne CC, Qu J, Inclan YF, Hawkins JS, Lu CHS, Silvis MR, Harden MM, Osadnik H, Peters JE, Engel JN, Dutton RJ, Grossman AD, Gross CA, Rosenberg OS
Nat Microbiol. 2019 Jan 7. pii: 10.1038/s41564-018-0327-z. doi: 10.1038/s41564-018-0327-z. PubMed Article
Peters JM, Koo BM, Patino R, Heussler GE, Hearne CC, Qu J, Inclan YF, Hawkins JS, Lu CHS, Silvis MR, Harden MM, Osadnik H, Peters JE, Engel JN, Dutton RJ, Grossman AD, Gross CA, Rosenberg OS
Nat Microbiol. 2019 Jan 7. pii: 10.1038/s41564-018-0327-z. doi: 10.1038/s41564-018-0327-z. PubMed Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 119239 | pJMP1039 | expresses Tn7 transposase |
| 119240 | pJMP1050 | construction intermediate |
| 119241 | pJMP1054 | construction intermediate |
| 119242 | pJMP1055 | construction intermediate |
| 119243 | pJMP1067 | rfp "test" strain |
| 119244 | pJMP1069 | rfp "test" strain |
| 119245 | pJMP1071 | rfp "test" strain |
| 119246 | pJMP1102 | knockdown phzA1 in P. aeruginosa |
| 119247 | pJMP1103 | knockdown phzM in P. aeruginosa |
| 119248 | pJMP1104 | knockdown pqsC in P. aeruginosa |
| 119249 | pJMP1106 | construction intermediate |
| 119250 | pJMP1159 | rfp "test" strain |
| 119251 | pJMP1161 | rfp "test" strain |
| 119252 | pJMP1170 | rfp "test" strain |
| 119253 | pJMP1171 | rfp "test" strain |
| 119254 | pJMP1183 | rfp "test" strain |
| 119255 | pJMP1185 | rfp "test" strain |
| 119256 | pJMP1187 | rfp "test" strain |
| 119257 | pJMP1189 | rfp "test" strain |
| 119258 | pJMP1217 | rfp "test" strain |
| 119259 | pJMP1219 | rfp "test" strain |
| 119260 | pJMP1221 | rfp "test" strain |
| 119261 | pJMP1223 | rfp "test" strain |
| 119262 | pJMP1237 | for cloning new sgRNAs |
| 119263 | pJMP1239 | knockdown folA in P. aeruginosa |
| 119264 | pJMP1263 | B. subtilis attTn7 site and flanking sequence cloned into pACYC |
| 119265 | pJMP1273 | E. coli attTn7 site with B. subtilis flanking sequence cloned into pACYC |
| 119266 | pJMP1274 | B. subtilis deltaTn7 site and flanking sequence cloned into pACYC |
| 119267 | pJMP1290 | construction intermediate |
| 119268 | pJMP1333 | rfp "test" strain |
| 119269 | pJMP1335 | rfp "test" strain |
| 119270 | pJMP1337 | for cloning new sgRNAs |
| 119271 | pJMP1339 | for cloning new sgRNAs |
| 119272 | pJMP1341 | control sgRNA in cells lacking rfp |
| 119273 | pJMP1344 | knockdown folA in E. aerogenes |
| 119274 | pJMP1346 | knockdown folA in K. pneumoniae |
| 119275 | pJMP1354 | for cloning new sgRNAs |
| 119276 | pJMP1356 | for cloning new sgRNAs |
| 119277 | pJMP1358 | for cloning new sgRNAs |
| 119278 | pJMP1360 | for cloning new sgRNAs |
| 119279 | pJMP1363 | for stabilizing ICE in the presence of rapI expression |