Multidimensional screening yields channelrhodopsin variants having improved photocurrent and order-of-magnitude reductions in calcium and proton currents.
Cho YK, Park D, Yang A, Chen F, Chuong AS, Klapoetke NC, Boyden ES
J Biol Chem. 2019 Jan 4. pii: RA118.006996. doi: 10.1074/jbc.RA118.006996. PubMed Article
Cho YK, Park D, Yang A, Chen F, Chuong AS, Klapoetke NC, Boyden ES
J Biol Chem. 2019 Jan 4. pii: RA118.006996. doi: 10.1074/jbc.RA118.006996. PubMed Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 123125 | pAAV-GFAP104-ChromeQ-mCherry | AAV-mediated expression of ChromeQ-mCherry under the GFAP promoter. |
| 123126 | pAAV-GFAP104-ChromeT-mCherry | AAV-mediated expression of ChromeT-mCherry under the GFAP promoter. |
| 123316 | pAAV-Syn-ChromeT-GFP | AAV-mediated expression of ChromeT-GFP under the Syn promoter. |
| 123317 | pAAV-Syn-ChromeQ-GFP | AAV-mediated expression of ChromeQ-GFP under the Syn promoter. |
| 153539 | pAAV-Syn-FLEX-rc [ChromeT-GFP] | AAV-mediated expression of ChromeT-GFP under the Syn promoter, in floxed/reversed (Cre-dependent) manner. |
| 153540 | pAAV-Syn-FLEX-rc [ChromeQ-GFP] | AAV-mediated expression of ChromeQ-GFP under the Syn promoter, in floxed/reversed (Cre-dependent) manner. |
| 153541 | pAAV-EF1α1.1-FLEX-rc [ChromeT-GFP] | AAV-mediated expression of ChromeT-GFP under the EF1α promoter (1.1kb short version), in floxed/reversed (Cre-dependent) manner. |
| 153542 | pAAV-EF1α1.1-FLEX-rc [ChromeQ-GFP] | AAV-mediated expression of ChromeQ-GFP under the EF1α promoter (1.1kb short version), in floxed/reversed (Cre-dependent) manner. |