Coupled Single-Cell CRISPR Screening and Epigenomic Profiling Reveals Causal Gene Regulatory Networks.

Rubin AJ, Parker KR, Satpathy AT, Qi Y, Wu B, Ong AJ, Mumbach MR, Ji AL, Kim DS, Cho SW, Zarnegar BJ, Greenleaf WJ, Chang HY, Khavari PA
Cell. 2018 Dec 11. pii: S0092-8674(18)31514-9. doi: 10.1016/j.cell.2018.11.022. PubMed Article

Plasmids from Article

ID	Plasmid	Purpose
117986	pLentiGuide	pLentiGuide is a gRNA insert lentiviral vector, with a stuffer sequence for BsmBI cloning of annealed oligos corresponding to sgRNA sequence. It has Puromycin resistance and Ampicilin resistance.
117987	pLex_Cas9	pLex_Cas9 is a single insert lentiviral vector expressing Cas9 driven by CMV promoter.