Multiplexed orthogonal genome editing and transcriptional activation by Cas12a.
Breinig M, Schweitzer AY, Herianto AM, Revia S, Schaefer L, Wendler L, Cobos Galvez A, Tschaharganeh DF
Nat Methods. 2019 Jan;16(1):51-54. doi: 10.1038/s41592-018-0262-1. Epub 2018 Dec 17.
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PubMed
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 122244 | pMB22 | Lentivirus delivery for stable expression of AsCas12a-VPR |
| 122245 | pMB34 | Lentivirus delivery for stable expression of LbCas12a-VPR |
| 122246 | pMB4 | Lentivirus delivery for stable expression of As crRNA, has AsDR; Cloning vector for expression of AsCpf1 crRNA. It contains BsmBI site for cloning. |
| 122247 | pMB30 | Lentivirus delivery for stable expression of Lb crRNA, has LbDR; Cloning vector for expression of LbCas12a crRNA. It contains BsmBI site for cloning. |