Dynamin-related protein 1 has membrane constricting and severing abilities sufficient for mitochondrial and peroxisomal fission.
Kamerkar SC, Kraus F, Sharpe AJ, Pucadyil TJ, Ryan MT
Nat Commun. 2018 Dec 7;9(1):5239. doi: 10.1038/s41467-018-07543-w.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 174421 | 6xHis(TEV)-dDrp1-StrepII | Expresses D. melanogaster Drp1 in bacteria |
| 174422 | 6xHis(TEV)-Drp1-StrepII | Expresses human Drp1 Isoform 3 in bacteria |
| 174423 | 6xHis(TEV)-Drp1(K38A)-StrepII | Expresses human Drp1 Isoform 3 with K38A mutation in bacteria |
| 174424 | 6xHis(Thrombin)-GFP(TEV)-Drp1-StrepII | Expresses human Drp1 Isoform 3 with EGFP at N-terminus in bacteria |
| 174425 | 6xHis(TEV)-Drp1(K557A,K560A)-StrepII | Expresses human Drp1 Isoform 3 with K557A, K560A mutations in bacteria |
| 174426 | 6xHis(TEV)-Drp1(K569A,K571A)-StrepII | Expresses human Drp1 Isoform 3 with K569A, K571A mutations in bacteria |
| 174427 | 6xHis(TEV)-yDrp1-StrepII | Expresses S. pombe Drp1 in bacteria |
| 174428 | Drp1-StrepII | Expresses human Drp1 Isoform 3 in bacteria |
| 174429 | Drp1-EGFP-StrepII | Expresses human Drp1 Isoform 3 with EGFP at C-terminus in bacteria |
| 174430 | Drp1(A395D)-StrepII | Expresses human Drp1 Isoform 3 with A395D mutation in bacteria |