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Functionally diverse type V CRISPR-Cas systems.
Yan WX, Hunnewell P, Alfonse LE, Carte JM, Keston-Smith E, Sothiselvam S, Garrity AJ, Chong S, Makarova KS, Koonin EV, Cheng DR, Scott DA
Science. 2019 Jan 4;363(6422):88-91. doi: 10.1126/science.aav7271. Epub 2018 Dec 6.
PubMed Article

Plasmids from Article

ID Plasmid Purpose  
120872pET28a-mH6-Cas12c1Expresses E. coli codon optimized Cas12c1 effector in the pET28a backbone.
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120873pET28a-mH6-Cas12c2Expresses E. coli codon optimized Cas12c2 effector in the pET28a backbone.
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120877pACYC-Cas12c2-noncodingExpresses concatenated noncoding sequences surrounding the CRISPR-Cas12c2 system in the native genomic loci; expresses the tracrRNA required for activity.
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120878pACYC-OspCas12c-noncodingExpresses concatenated noncoding sequences surrounding the CRISPR-OspCas12c system in the native genomic loci; expresses the tracrRNA required for activity.
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120879pET28a-mH6-Cas12g1Expresses E. coli codon optimized Cas12g1 effector in the pET28a backbone.
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120880pACYC-Cas12g1-noncodingExpresses concatenated noncoding sequences surrounding the CRISPR-Cas12g1 system in the native genomic loci; expresses the tracrRNA required for activity.
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120882pET28a-mH6-Cas12i1Expresses E. coli codon optimized Cas12i1 effector in the pET28a backbone.
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120883pET28a-mH6-Cas12i2Expresses E. coli codon optimized Cas12i2 effector in the pET28a backbone.
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Antibodies from Article