Multiple-step chromosomal integration of divided segments from a large DNA fragment via CRISPR/Cas9 in Escherichia coli.

Li Y, Yan F, Wu H, Li G, Han Y, Ma Q, Fan X, Zhang C, Xu Q, Xie X, Chen N
J Ind Microbiol Biotechnol. 2019 Jan;46(1):81-90. doi: 10.1007/s10295-018-2114-5. Epub 2018 Nov 23. PubMed Article

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