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A Forster resonance energy transfer sensor for live-cell imaging of mitogen-activated protein kinase activity in Arabidopsis.

Zaman N, Seitz K, Kabir M, George-Schreder LS, Shepstone I, Liu Y, Zhang S, Krysan PJ
Plant J. 2018 Nov 16. doi: 10.1111/tpj.14164. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
118935pET-SOMAUsed to express the SOMA FRET sensor in E. coli.
118936pET-SOMA-T679AUsed to express the T679A mutant form of the SOMA FRET sensor in E. coli.
118937pSOMA-NLSUsed to express the SOMA FRET sensor with a nuclear localization signal in Arabidopsis thaliana
118938pSOMA-T679A-NLSUsed to express the T679A mutant form of the SOMA FRET sensor with a nuclear localization signal in Arabidopsis thaliana
118939pSOMA-NESUsed to express the SOMA FRET sensor with a nuclear exclusion signal in Arabidopsis thaliana
118940pSOMA-T679A-NESUsed to express the T679A mutant form of the SOMA FRET sensor with a nuclear exclusion signal in Arabidopsis thaliana

Antibodies from Article