Skip to main content
This website uses cookies to ensure you get the best experience. By continuing to use this site, you agree to the use of cookies.

Please note: Your browser does not support the features used on Addgene's website. You may not be able to create an account or request plasmids through this website until you upgrade your browser. Learn more

Please note: Your browser does not fully support some of the features used on Addgene's website. If you run into any problems registering, depositing, or ordering please contact us at [email protected]. Learn more

Addgene
Structural Basis for Cholesterol Transport-like Activity of the Hedgehog Receptor Patched.
Zhang Y, Bulkley DP, Xin Y, Roberts KJ, Asarnow DE, Sharma A, Myers BR, Cho W, Cheng Y, Beachy PA
Cell. 2018 Nov 15;175(5):1352-1364.e14. doi: 10.1016/j.cell.2018.10.026. Epub 2018 Nov 8.
PubMed Article

Plasmids from Article

ID Plasmid Purpose
120886pVLAD6-mmPtch1-B-SBPBacMam vector for Ptch1-B expression
120887pVLAD6-mmPtch1-B-NNQ-TEV-SBPBacMam vector for Ptch1-B NNQ variant expression
120888pcDNA-h-mmPtch1-BExpresses Ptch1-B for function assays
120889pcDNA-h-mmPtch1-FLExpresses wildtype Ptch1 for function assays
120902pcDNA-h-mmPtch1-B-HAExpresses Ptch1-B for function assays
120903pcDNA-h-mmPtch1-B-HA-GFPExpresses Ptch1-B for function assays
120904pcDNA-h-mmPtch1-B-Y233C-HAExpresses Ptch1-B (Y233C) for function assays
120905pcDNA-h-mmPtch1-B-L234C-HAExpresses Ptch1-B (L234C)
120906pcDNA-h-mmPtch1-B-L235C-HAExpresses mmPtch1-B (L235C)
120907pcDNA-h-mmPtch1-B-I766FV111F-HA-GFPExpresses Ptch1-B (I766FV111F)
120908pcDNA-h-mmPtch1-B-V111FL114FW115A-HA-GFPExpresses Ptch1-B (V111FL114FW115A)
120909pcDNA-h-mmPtch1-B-I766FV111FL114F-HA-GFPExpresses Ptch1-B (I766FV111FL114F)
120910pcDNA-h-mmPtch1-B-HA-TagBFPExpresses BFP tagged Ptch1-B
120911pcDNA-h-mmPtch1-B-V111FL114FI766F-HA-TagBFPExpresses BFP tagged Ptch1-B (V111FL114FI766F)
120921pcDNA-h-mmPtch1-FL-TagBFPExpresses BFP tagged Ptch1
121135pHTSHP-Smt-IIShhNExpresses N-terminal SUMO tagged II-ShhN in bacteria
121136pHTSHP-ShhN-CysExpresses ShhN with a C-terminal cysteine residues in bacteria
121164pcDNA-h-mmPtch1-B-NNQ-HA-GFPExpresses mmPtch1-B NNQ variant

Antibodies from Article