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Ubiquitin-Independent Disassembly by a p97 AAA-ATPase Complex Drives PP1 Holoenzyme Formation.
Weith M, Seiler J, van den Boom J, Kracht M, Hulsmann J, Primorac I, Del Pino Garcia J, Kaschani F, Kaiser M, Musacchio A, Bollen M, Meyer H
Mol Cell. 2018 Oct 11. pii: S1097-2765(18)30791-3. doi: 10.1016/j.molcel.2018.09.020.
PubMed Article

Plasmids from Article

ID Plasmid Purpose  
169020pET15b His-p97Expresses His-p97 in bacteria
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169021pET15b p97-Strep-His E314AmbExpresses p97-Strep-His E314Amb (incorporates noncanonical amino acid at position 314 via amber suppression) in bacteria
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169022pET15b p97-His D592AmbExpresses p97-His D592Amb (incorporates noncanonical amino acid at position 592 via amber suppression) in bacteria
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Antibodies from Article