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CRISPR-Mediated Programmable 3D Genome Positioning and Nuclear Organization.
Wang H, Xu X, Nguyen CM, Liu Y, Gao Y, Lin X, Daley T, Kipniss NH, La Russa M, Qi LS
Cell. 2018 Nov 15;175(5):1405-1417.e14. doi: 10.1016/j.cell.2018.09.013. Epub 2018 Oct 11.
PubMed Article

Plasmids from Article

ID Plasmid Purpose  
121513pHR-PGK-ABI-dCas9-P2A-mcherryexpression of AB1-fused-dcas9 plasmids
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121532pHR-SFFV-PYL1-sfGFP-Emerinexpression of PYL-SfGFP-Emerin, lentivirus vector
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122177pHR-PGK-PYL1-sfGFP-Coilinexpression of PYL1-SfGFP-Coilin, lentivirus vector
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167011pHR-TRE3G-PYL1-sfGFP-PMLexpression of PYL1-SfGFP-PML, lentivirus vector
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169446pHR-PGK-ABI-tagBFP-dCas9expression of AB1-fused-dcas9 plasmids
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169448pHR-TRE3G-dCas9-HaloTagexpression of dCas9-HaloTag
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169449pHR-U6-sgTel-CMV-puro-P2A-mCherry-TRF1expression of TRF1-mcherrry and sgRNA targeting telomeres
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169450pHR-U6-sgRNA-CMV-purobackbone for sgRNA expression
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169451pHR-TRE3G-dCas9-EGFP-HaloTagexpression of dCas9-EGFP-HaloTag
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Antibodies from Article