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High fidelity CRISPR/Cas9 increases precise monoallelic and biallelic editing events in primordial germ cells.
Idoko-Akoh A, Taylor L, Sang HM, McGrew MJ
Sci Rep. 2018 Oct 11;8(1):15126. doi: 10.1038/s41598-018-33244-x.
PubMed Article

Plasmids from Article

ID Plasmid Purpose  
118632HF-PX459 (V2)Plasmid encoding SpCas9-HF1, a single guide RNA and puromycin resistance
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Antibodies from Article