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Primate immunodeficiency virus proteins Vpx and Vpr counteract transcriptional repression of proviruses by the HUSH complex.
Yurkovetskiy L, Guney MH, Kim K, Goh SL, McCauley S, Dauphin A, Diehl WE, Luban J
Nat Microbiol. 2018 Dec;3(12):1354-1361. doi: 10.1038/s41564-018-0256-x. Epub 2018 Oct 8. PubMed Article

Plasmids from Article

ID Plasmid Purpose
115803pscALPS gag-gfp BlastiExpress gag-gfp fusion driven by SFFV promoter
115804pscALPS gfp BlastiExpress gfp driven by SFFV promoter
115805pecALPS gag-gfp BlastiExpress gag-gfp fusion driven by EIF1a promoter
115806ptcALPS gag-gfp BlastiExpress gag-gfp fusion driven by TK promoter
115807pscALPS gag-gfp/deltavpxSFFV promoter expresses gag-gfp fusion with no ORF after CypA promoter
115808pscALPS gag-gfp/vpxSFFV promoter expresses gag-gfp fusion with CypA promoter driving expression of SIVMAC251 vpx
115809HIV-1 LTR-gfpHIV-1 LTR driven reporter vector that retains complete LTRs, tat, and rev, but has a frameshift mutation in env, an ngfr reporter gene in place of nef, and gfp in place of gag, pol, vif, and vpr
115810pscALPS- SIV-MAC251 vpx Encodes codon optimized 3xFLAG-VpxMAC251 and puromycin resistance protein
115811pscALPS SIV-MND2 vpxEncodes codon optimized 3xFLAG-SIVMND2 Vpx (AY159322) and puromycin resistance protein
115812pscALPS-SIV-RCM vpxEncodes codon optimized 3xFLAG-SIVRCM Vpx (AF349680) and puromycin resistance protein
115813pscALPS-SIV-MNE vpxEncodes codon optimized 3xFLAG-SIVMNE Vpx (U79412) and puromycin resistance protein
115814pscALPS-SIV-DRL vpxEncodes codon optimized 3xFLAG-SIVDRL Vpx (KM378563) and puromycin resistance protein
115815pscALPS-SIV-AGI vpxEncodes codon optimized 3xFLAG-SIVAGI Vpx (HM803690) and puromycin resistance protein
115816pscALPS- HIV2-ROD vpxEncodes codon optimized 3xFLAG-HIV2ROD Vpx (M15390) and puromycin resistance protein
115817pscALPS-SIV-MND1 vprEncodes codon optimized 3xFLAG-SIVMND1 Vpr (M27470) and puromycin resistance protein
115818pscALPS-SIV-LST vprEncodes codon optimized 3xFLAG-SIVLST Vpr (AF188116) and puromycin resistance protein
115819pscALPS-SIV-AGM-TAN1 vprEncodes codon optimized 3xFLAG-SIVAGM Vpr (TAN1) (U58991) with repaired premature stop codon and puromycin resistance protein
115820pscALPS-SIV-TAL vprEncodes codon optimized 3xFLAG-SIVTAL Vpr (AM182197) and puromycin resistance protein
115821pscALPS-SIV-SYK vprEncodes codon optimized 3xFLAG-SIVSYK Vpr (L06042) and puromycin resistance protein
115822pscALPS-SIV-SAB-92018 vprEncodes codon optimized 3xFLAG-SIVSAB Vpr (HQ378594) and puromycin resistance protein
115823pscALPS-SIV-DEB vprEncodes codon optimized 3xFLAG-SIVDEB Vpr (FJ919724) and puromycin resistance protein
115824pscALPS-SIV-ASC vprEncodes codon optimized 3xFLAG-SIVASC Vpr (KJ461715) and puromycin resistance protein
115825pscALPS-SIV-AGM-VER9063 vprEncodes codon optimized 3xFLAG-SIVAGM Vpr (Ver 9063) (L40990) and Puro resistance marker
115826pscALPS-SIV-AGM-VERAGM3 vprEncodes codon optimized 3xFLAG-SIVAGM Vpr (Ver AGM3) (M30931) and puromycin resistance protein
115827pscALPS-SIV-GRV vprEncodes codon optimized 3xFLAG-SIVGRV Vpr (M66437) and puromycin resistance protein
115828pscALPS-SIV-AGM-MAL vprEncodes codon optimized 3xFLAG-SIVAGM Vpr (MAL_ZMB) (LC114462) and puromycin resistance protein
115829pscALPS-SIV-DEN vprEncodes codon optimized 3xFLAG SIVDEN Vpr (AJ580407) and puromycin resistance protein
115830pscALPS-SIV-GSN-CN71 vprEncodes codon optimized 3xFLAG SIVGSN-CN71 Vpr (AF468658) and puromycin resistance protein
115831pscALPS-SIV-GSN-CN166 vprEncodes codon optimized 3xFLAG SIVGSN-CN166 Vpr (AF468659) and puromycin resistance protein
115832pscALPS-SIV-SUN-K08 vprEncodes codon optimized 3xFLAG-SIVSUN Vpr (FR751162) and puromycin resistance protein
115833pscALPS-SIV-CPZ-TAN3 vprEncodes codon optimized 3xFLAG-SIVCPZ-TAN3 Vpr (DQ374658) and puromycin resistance protein
115834pscALPS-SIV-CPZ-LB7 vprEncodes codon optimized 3xFLAG-SIVCPZ-LB7 Vpr (DQ373064) and puromycin resistance protein
115835pscALPS-SIV-GOR -vprEncodes codon optimized 3xFLAG-SIVGOR Vpr (FJ424871) and puromycin resistance protein
115836pscALPS-HIV-1 Group O-vprEncodes codon optimized 3xFLAG-HIV1 Group O Vpr (L20571) and puromycin resistance protein
115837pscALPS-HIV-1 Group P-vprEncodes codon optimized 3xFLAG-HIV1 Group P Vpr (HQ179987) and puromycin resistance protein
115838pscALPS-SIV-RCM-vprEncodes codon optimized 3xFLAG-SIVRCM Vpr (HM803689) and puromycin resistance protein
115839pscALPS-SIV-WRC-vprEncodes codon optimized 3xFLAG-SIVWRC Vpr (AM713177) and puromycin resistance protein
115840pscALPS-SIV-COL-BWC01 vprEncodes codon optimized 3xFLAG-SIVCOLVpr (KF214240) and puromycin resistance protein
115841pscALPS-SIV-MUS2-1246-vprEncodes codon optimized 3xFLAG-SIVMUS21246 Vpr (EF070329) and puromycin resistance protein
115842pcDNA3.1 FLAG-SIV-MAC251-vpxEncodes codon optimized FLAG tagged SIVMAC251-Vpx
115843pcDNA3.1 FLAG- SIV-MAC251-vpx-Q76AEncodes codon optimized FLAG tagged SIVMAC251-Vpx-Q76A mutant
115844pcDNA3.1 FLAG-SIV-RCM-vpxEncodes codon optimized FLAG tagged SIVRCM-Vpx
115845pcDNA3.1 HA-FAM208AEncodes codon optimized HA tagged FAM208A
115846pAPM-D4- miR30-L1221Control shRNA KD vector
115847pAPM-D4 miR30-AGO1 ts3AGO1 knockdown
115848pAPM-D4 miR30-HDAC1 ts2HDAC1 knockdown
115849pAPM-D4 miR30-HDAC1 ts3HDAC1 knockdown
115850pAPM-D4 miR30-AGO1 ts1AGO1 knockdown
115851pAPM-D4 miR30-AGO1 ts2AGO1 knockdown
115852pAPM-D4 miR30-HDAC1 ts1HDAC1 knockdown
115853pAPM-D4 miR30-AGO2 ts1AGO2 knockdown
115854pAPM-D4 miR30-AGO2 ts2AGO2 knockdown
115855pAPM-D4 miR30-AGO2 ts3AGO2 knockdown
115856pAPM-D4 miR30-AGO3 ts1AGO3 knockdown
115857pAPM-D4 miR30-AGO3 ts2AGO3 knockdown
115858pAPM-D4 miR30-AGO3 ts3AGO3 knockdown
115859pAPM-D4 miR30-SETDB1 ts1SETDB1 knockdown
115860pAPM-D4 miR30-SETDB1 ts2SETDB1 knockdown
115861pAPM-D4 miR30-SETDB1 ts3SETDB1 knockdown
115862pAPM-D4 miR30-TRIM28 ts1TRIM28 knockdown
115864pAPM-D4 miR30-TRIM28 ts3TRIM28 knockdown
115865pAPM-D4 miR30-DNMT3a ts1DNMT3a knockdown
115866pAPM-D4 miR30-DNMT3a ts2DNMT3a knockdown
115867pAPM-D4 miR30-DNMT3a ts3DNMT3a knockdown
115868pAPM-D4 miR30-PIWIL2 ts1PIWIL2 knockdown
115869pAPM-D4 miR30-PIWIL2 ts3PIWIL2 knockdown
115870pAPM-D4 miR30-PIWIL2 ts2PIWIL2 knockdown
115871pAPM-D4 miR30-FAM208A ts1FAM208a knockdown
115872pAPM-D4 miR30-FAM208A ts2FAM208a knockdown
115873pAPM-D4 miR30-FAM208A ts3FAM208a knockdown
115874pAPM-D4 miR30-MPHOSPH8 ts1MPHOSPH8 knockdown
115875pAPM-D4 miR30-MPHOSPH8 ts2MPHOSPH8 knockdown
115876pAPM-D4 miR30-MPHOSPH8 ts3MPHOSPH8 knockdown
115877pAPM-D4 miR30-PPHLN1 ts1PPHLN1 knockdown
115878pAPM-D4 miR30-PPHLN1 ts2PPHLN1 knockdown
115879pAPM-D4 miR30-PPHLN1 ts3PPHLN1 knockdown
115880pAPM-D4 miR30-SUV39h1 ts1SUV39h1 knockdown
115881pAPM-D4 miR30-SUV39h1 ts2SUV39h1 knockdown
115882pAPM-D4 miR30-SUV39h1 ts3SUV39h1 knockdown
115883pAPM-D4 miR30-SUV39h2 ts1SUV39h2 knockdown
115884pAPM-D4 miR30-SUV39h2 ts2SUV39h2 knockdown
115885pAPM-D4 miR30-SUV39h2 ts3SUV39h2 knockdown
115886pAPM-D4 miR30-MORC2 ts1MORC2 knockdown
115887pAPM-D4 miR30-MORC2 ts2MORC2 knockdown
115888pAPM-D4 miR30-MORC2 ts3MORC2 knockdown
128504pscALPS_puroEncodes puromycin resistance protein
128505pAPM D4 BsmBI Myd88 stufferpAPM knockdown vector with Myd88 stuffer sequence

Antibodies from Article