Efficient C-to-T base editing in plants using a fusion of nCas9 and human APOBEC3A.
Zong Y, Song Q, Li C, Jin S, Zhang D, Wang Y, Qiu JL, Gao C
Nat Biotechnol. 2018 Oct 1. pii: nbt.4261. doi: 10.1038/nbt.4261. PubMed Article
Zong Y, Song Q, Li C, Jin S, Zhang D, Wang Y, Qiu JL, Gao C
Nat Biotechnol. 2018 Oct 1. pii: nbt.4261. doi: 10.1038/nbt.4261. PubMed Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 119768 | A3A-PBE | Conversion of C to T in wheat plants or monocotyledons protoplasts |
| 119769 | A3A-Gam | Conversion of C to T monocotyledons protoplasts |
| 119770 | A3A-PBE-ΔUGI | Conversion of C to T in wheat plants or monocotyledons protoplasts without uracil glycosylase inhibitor (UGI) |
| 119771 | pET42b-A3A-PBE-ΔUGI | To express the recombinant APOBEC3A in vitro |
| 119772 | p35S-PBE | Conversion of C to T in potato |
| 119773 | p35S-A3A-PBE | Conversion of C to T in potato |
| 119774 | pH-A3A-PBE | Conversion of C to T in rice plants transformation |
| 119775 | pAtU6-sgRNA | Expression of a target sgRNA in potato |