Methanomethylophilus alvus Mx1201 provides basis for mutual orthogonal pyrrolysyl tRNA/aminoacyl-tRNA synthetase pairs in mammalian cells.
Meineke B, Heimgartner J, Lafranchi L, Elsasser SJ
ACS Chem Biol. 2018 Sep 27. doi: 10.1021/acschembio.8b00571. PubMed Article
Meineke B, Heimgartner J, Lafranchi L, Elsasser SJ
ACS Chem Biol. 2018 Sep 27. doi: 10.1021/acschembio.8b00571. PubMed Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 140008 | pAS | Plasmid with 4xMmaPylT cassette to receive genes of interest for amber suppression; for transient or stable piggyBac-mediated integration |
| 140009 | pAS_4xMma PylT_FLAG-Mma PylRS | Plasmid with 4xMmaPylT cassette and Mma PylRS for amber suppression; for transient or stable piggyBac-mediated integration |
| 140010 | pAS_4xMma PylT(UUA)_FLAG-AcKRS | Plasmid with 4xMmaP cassette and Mma PylRS for ochre (TAA) suppression; for transient or stable piggyBac-mediated integration |
| 140011 | pAS_4xMx PylT_FLAG-Mx PylRS | Plasmid with 4xMx PylT cassette and MX1201 PylRS for amber suppression; for transient or stable piggyBac-mediated integration |
| 140012 | pAS_4xMx PylT_FLAG-Mx PylRS YA | Plasmid with 4xMx PylT cassette and MX1201 PylRS for amber suppression; for transient or stable piggyBac-mediated integration |
| 140013 | pAS_4xMx PylT C41CA_FLAG_Mx PylRS | Plasmid with 4xMx PylT C41CA cassette and MX1201 PylRS for amber suppression; for transient or stable piggyBac-mediated integration |
| 140014 | pAS_4xMx PylT C41CA_FLAG_Mx PylRS YA | Plasmid with 4xMx PylT C41CA cassette and MX1201 PylRS for amber suppression; for transient or stable piggyBac-mediated integration |
| 140015 | pAS_4xMma PylT_GFP 150TAG 6xHis | Plasmid with 4xMmaPylT cassette and amber suppression reporter sfGFP 150 TAG stop; for transient transfection or stable piggyBac-mediated integration |
| 140016 | pAS_4xMx PylT_GFP 150TAG | Plasmid with 4xMxPylT cassette and amber suppression reporter sfGFP 150 TAG stop; for transient transfection or stable piggyBac-mediated integration |
| 140017 | pAS_4xMx PylT C41CA_GFP 150TAG | Plasmid with 4xMxPylT C41CA cassette and amber suppression reporter sfGFP 150 TAG stop; for transient transfection or stable piggyBac-mediated integration |
| 140023 | pAS_4xMma PylT_FLAG-Mma PylRS AF | Plasmid with 4xMmaPylT cassette and MmaPylRS AF for amber suppression; for transient or stable piggyBac-mediated integration |
| 154762 | pAS_FLAG-Mma PylRS | plasmid with Mma PylRS for amber suppression in transient or stable, piggybac-mediated, integration |
| 154764 | pAS_FLAG-Mx1201 PylRS | plasmid with Mx1201 PylRS for amber suppression in transient or stable, piggybac-mediated, integration |
| 154765 | pAS_sfGPF 150TAG | plasmid with amber suppression reporter sfGFP 150 TAG stop, for transient transfection or stable, piggybac-mediated, integration |
| 154766 | pAS_4xMma PylT sfGPF 150TAG | plasmid with 4xMma PylT cassette and amber suppression reporter sfGFP 150 TAG stop, for transient transfection or stable, piggybac-mediated, integration |
| 154782 | pUC_h7SK Mma PylT (CUA) | plasmid with single copy of Mma PylT (CUA anticodon) |