Efficient proximity labeling in living cells and organisms with TurboID.
Branon TC, Bosch JA, Sanchez AD, Udeshi ND, Svinkina T, Carr SA, Feldman JL, Perrimon N, Ting AY
Nat Biotechnol. 2018 Aug 20. pii: nbt.4201. doi: 10.1038/nbt.4201. PubMed Article
Branon TC, Bosch JA, Sanchez AD, Udeshi ND, Svinkina T, Carr SA, Feldman JL, Perrimon N, Ting AY
Nat Biotechnol. 2018 Aug 20. pii: nbt.4201. doi: 10.1038/nbt.4201. PubMed Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 107167 | TurboID-V5_pRS415 | expresses V5-tagged TurboID in the yeast cytosol |
| 107168 | miniTurbo-V5_pRS415 | expresses V5-tagged miniTurbo in the yeast cytosol |
| 107170 | V5-miniTurbo-NES_pCDNA3 | expresses V5-tagged miniTurbo in the mammalian cytosol |
| 107171 | 3xHA-TurboID-NLS_pCDNA3 | expresses 3xHA-tagged TurboID in the mammalian nucleus |
| 107172 | 3xHA-miniTurbo-NLS_pCDNA3 | expresses 3xHA-tagged miniTurbo in the mammalian nucleus |
| 107173 | C1(1-29)-TurboID-V5_pCDNA3 | expresses V5-tagged TurboID on the mammalian ER membrane |
| 107174 | C1(1-29)-miniTurbo-V5_pCDNA3 | expresses V5-tagged miniTurbo on the mammalian ER membrane |
| 107175 | C1(1-29)-TurboID-V5_pLX304 | expresses V5-tagged TurboID on the mammalian ER membrane, lentiviral vector |
| 107176 | C1(1-29)-miniTurbo-V5_pLX304 | expresses V5-tagged miniTurbo on the mammalian ER membrane, lentiviral vector |
| 107177 | TurboID-His6_pET21a | expresses His6-tagged TurboID in bacteria |
| 107178 | miniTurbo-His6_pET21a | expresses His6-tagged miniTurbo in bacteria |
| 118220 | 3xHA-TurboID_pAS31 | expresses 3xHA-tagged TurboID in C. elegans intestine |
| 118222 | 3xHA-miniTurbo_pAS32 | expresses 3xHA-tagged miniTurbo in C. elegans intestine |
| 118223 | 3xHA-BioID_pAS28 | expresses 3xHA-tagged BioID in C. elegans intestine |