Cas9-mediated allelic exchange repairs compound heterozygous recessive mutations in mice.
Wang D, Li J, Song CQ, Tran K, Mou H, Wu PH, Tai PWL, Mendonca CA, Ren L, Wang BY, Su Q, Gessler DJ, Zamore PD, Xue W, Gao G
Nat Biotechnol. 2018 Oct;36(9):839-842. doi: 10.1038/nbt.4219. Epub 2018 Aug 13. PubMed Article
Wang D, Li J, Song CQ, Tran K, Mou H, Wu PH, Tai PWL, Mendonca CA, Ren L, Wang BY, Su Q, Gessler DJ, Zamore PD, Xue W, Gao G
Nat Biotechnol. 2018 Oct;36(9):839-842. doi: 10.1038/nbt.4219. Epub 2018 Aug 13. PubMed Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 121507 | pAAV.pU1a-SpCas9 | Expresses codon-optimized SpCas9 in mammalian cells. |
| 121508 | pAAVsc.U6-sgFah.CMV/CB-EGFP | Expresses sgRNA targeting mouse Fah intron 7 (sgFah). |
| 121509 | pAAVsc.U6-sgFah.Intron9.CMV/CB-EGFP | Expresses sgRNA targeting mouse Fah intron 9 (sgFah.Intron9). |
| 121510 | pAAVsc.U6-sgAspa.CMV/CB-EGFP | Expresses sgRNA targeting mouse Aspa intron 2 (sgAspa). |
| 121511 | pAAVsc.U6-sgIdua.CMV/CB-EGFP | Expresses sgRNA targeting mouse Idua intron 9 (sgIdua). |
| 121512 | pAAVsc.U6-sgGTTG.CMV/CB-Gluc | Expresses sgRNA targeting the intron in the MADM alleles (GT and TG). |