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Cryo-EM of the dynamin polymer assembled on lipid membrane.
Kong L, Sochacki KA, Wang H, Fang S, Canagarajah B, Kehr AD, Rice WJ, Strub MP, Taraska JW, Hinshaw JE
Nature. 2018 Aug;560(7717):258-262. doi: 10.1038/s41586-018-0378-6. Epub 2018 Aug 1. PubMed Article

Plasmids from Article

ID Plasmid Purpose
112102pEGFP-N1 Dynamin1 P294AMammalian expression of GFP-tagged Dynamin protein.
112103pEGFP-N1 Dynamin1 R290A D291A T292AMammalian expression of GFP-tagged Dynamin protein.
112104pEGFP-N1 Dynamin1 T292A L293A P294AMammalian expression plasmid of GFP-tagged Dynamin protein.
112105pEGFP-N1 Dynamin1 L330R Q334R L702RMammalian expression plasmid of GFP-tagged Dynamin protein.
112106pEGFP-N1 Dynamin1 T78R L84R T92R V118RMammalian expression plasmid of GFP-tagged Dynamin protein.
112107pEGFP-N1 Dynamin1 D406A M407AMammalian expression plasmid of GFP-tagged Dynamin protein.
112108pEGFP-N1 Dynamin1 D406A M407A F409AMammalian expression plasmid of GFP-tagged Dynamin protein.
112109pEGFP-N1 Dynamin1 D406R M407RMammalian expression plasmid of GFP-tagged Dynamin protein.
112110pEGFP-N1 Dynamin1 D406R M407R F409AMammalian expression plasmid of GFP-tagged Dynamin protein.
112111pEGFP-N1 Dynamin1 D406R M407R T488WMammalian expression plasmid of GFP-tagged Dynamin protein.
120313pEGFP-N1 Dynamin1 wtMammalian expression plasmid of GFP-tagged Dynamin protein.

Antibodies from Article