Reprogramming human T cell function and specificity with non-viral genome targeting.
Roth TL, Puig-Saus C, Yu R, Shifrut E, Carnevale J, Li PJ, Hiatt J, Saco J, Krystofinski P, Li H, Tobin V, Nguyen DN, Lee MR, Putnam AL, Ferris AL, Chen JW, Schickel JN, Pellerin L, Carmody D, Alkorta-Aranburu G, Del Gaudio D, Matsumoto H, Morell M, Mao Y, Cho M, Quadros RM, Gurumurthy CB, Smith B, Haugwitz M, Hughes SH, Weissman JS, Schumann K, Esensten JH, May AP, Ashworth A, Kupfer GM, Greeley SAW, Bacchetta R, Meffre E, Roncarolo MG, Romberg N, Herold KC, Ribas A, Leonetti MD, Marson A
Nature. 2018 Jul 11. pii: 10.1038/s41586-018-0326-5. doi: 10.1038/s41586-018-0326-5. PubMed Article
Roth TL, Puig-Saus C, Yu R, Shifrut E, Carnevale J, Li PJ, Hiatt J, Saco J, Krystofinski P, Li H, Tobin V, Nguyen DN, Lee MR, Putnam AL, Ferris AL, Chen JW, Schickel JN, Pellerin L, Carmody D, Alkorta-Aranburu G, Del Gaudio D, Matsumoto H, Morell M, Mao Y, Cho M, Quadros RM, Gurumurthy CB, Smith B, Haugwitz M, Hughes SH, Weissman JS, Schumann K, Esensten JH, May AP, Ashworth A, Kupfer GM, Greeley SAW, Bacchetta R, Meffre E, Roncarolo MG, Romberg N, Herold KC, Ribas A, Leonetti MD, Marson A
Nature. 2018 Jul 11. pii: 10.1038/s41586-018-0326-5. doi: 10.1038/s41586-018-0326-5. PubMed Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 112012 | RAB11A-GFP HDRT Source (pTR 143) | DNA sequence source for amplifying an HDR template to tag endogenous human RAB11A gene with GFP |
| 112013 | RAB11A-mCherry HDRT Source (pTR 144) | DNA sequence source for amplifying an HDR template to tag endogenous human RAB11A gene with mCherry |
| 112014 | RAB11A-BFP HDRT Source (pTR 145) | DNA sequence source for amplifying an HDR template to tag endogenous human RAB11A gene with BFP |
| 112015 | BATF-GFP HDRT Source (pTR 146) | DNA sequence source for amplifying an HDR template to tag endogenous human BATF gene with GFP |
| 112016 | CLTA-GFP HDRT Source (pTR 153) | DNA sequence source for amplifying an HDR template to tag endogenous human CLTA gene with GFP |
| 112017 | CLTA-mCherry HDRT Source (pTR 177) | DNA sequence source for amplifying an HDR template to tag endogenous human CLTA gene with mCherry |
| 112018 | CD4-C-GFP HDRT Source (pTR 152) | DNA sequence source for amplifying an HDR template to tag endogenous human CD4 gene with GFP |
| 112019 | CD4-C-mCherry HDRT Source (pTR 175) | DNA sequence source for amplifying an HDR template to tag endogenous human CD4 gene with mCherry |
| 112020 | CD4-C-BFP HDRT Source (pTR 176) | DNA sequence source for amplifying an HDR template to tag endogenous human CD4 gene with BFP |
| 112021 | NYESO beta/alpha into TRAC HDRT Source (pTR 169) | DNA sequence source for amplifying an HDR template to replace endogenous human TCR with 1G4 NYESO TCR at TCR-alpha |
| 112022 | NYESO alpha/beta into TRBC1 HDRT Source (pTR 262) | DNA sequence source for amplifying an HDR template to replace endogenous human TCR with 1G4 NYESO TCR at TCR-beta |
| 112023 | NYESO alpha into TRAC HDRT Source (pTR 223) | DNA sequence source for amplifying an HDR template to replace endogenous human TCR-alpha with 1G4 NYESO TCR-alpha |
| 112024 | NYESO beta into TRBC1 HDRT Source (pTR 277) | DNA sequence source for amplifying an HDR template to replace endogenous human TCR-beta with 1G4 NYESO TCR-beta |