Chemogenetic control of gene expression and cell signaling with antiviral drugs.
Tague EP, Dotson HL, Tunney SN, Sloas DC, Ngo JT
Nat Methods. 2018 Jul;15(7):519-522. doi: 10.1038/s41592-018-0042-y. Epub 2018 Jul 2.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 112244 | dCas9-NS3-NLS/VPR | Encodes the drug-preservable Cas9-NS3-NLS/VPR, which can be used to activate gene expression when combined with an NS3 inhibitor and appropriately designed sgRNA. |
| 112279 | ss-SNAP-TMD-NS3-Gal4 | Encodes the membrane-targeted TMD-NS3-Gal4, which exhibits drug-inducible transcription "turn-off" activity. SNAP-tag is fused to the extracellular region of the encoded protein construct. |
| 112627 | Gal4DB-NS3-VP64-p65_IRES_mCherry | Encodes the drug-activated transcription factor Gal4DB-NS3-VP64-p65 along with IRES mCherry as a fluorescent marker. |
| 112628 | rTetR-NS3-VP64-p65_IRES_mCherry | Encodes the drug-activated transcription factor rTetR-NS3-VP64-p65 along with IRES mCherry as a fluorescent marker. |