An optogenetic approach to control protein localization during embryogenesis of the sea urchin.
Uchida A, Yajima M
Dev Biol. 2018 Sep 1;441(1):19-30. doi: 10.1016/j.ydbio.2018.06.015. Epub 2018 Jun 26.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 135597 | pSP64-mCherry | Expression of mCherry mRNA for microinjection into S. purpuratus |
| 135598 | pSP64-membrane-mCherry | Expression of membrane-mCherry mRNA for microinjection for visualization of membrane in S. pupuratus |
| 135599 | pSP64-membrane-mCherry-LOV | Expression of membrane-mCherry-LOV mRNA for microinjection for use of optogenetics in S. purpuratus |
| 135600 | pSP64-EMTB-GFP-ePDZ | Expression of EMTB-GFP-ePDZ mRNA for microinjection for use of optogenetics in S. purpuratus |
| 135601 | pSP64-Lifeact-GFP | Expression of Lifeact-GFP mRNA for microinjection for visualization of actin in S. purpuratus |
| 135602 | pSP64-Lifeact-GFP-ePDZ | Expression of Lifeact-GFP-ePDZ mRNA for microinjection to be used for optogenetics in S. purpuratus |
| 135603 | pSP64-GFP-ePDZ-CAAX | Expression of GFP-ePDZ-CAAX mRNA for microinjection to be used for optogenetics in S. purpuratus |
| 135604 | pCS2-H2B-mCherry | Expression of H2B-mCherry mRNA for microinjection to visualize Histone 2B protein dynamics in S. purpuratus |
| 135605 | pSP64-H2B-GFP | Expression of H2B-GFP mRNA for microinjection to visualize Histone 2B protein dynamics in S. purpuratus |