Addgene: Metabolic engineering of a carbapenem antibiotic synthesis pathway in Escherichia coli.

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Greg Bokinsky
Shomar et al

Metabolic engineering of a carbapenem antibiotic synthesis pathway in Escherichia coli.
Shomar H, Gontier S, van den Broek NJF, Tejeda Mora H, Noga MJ, Hagedoorn PL, Bokinsky G
Nat Chem Biol. 2018 Aug;14(8):794-800. doi: 10.1038/s41589-018-0084-6. Epub 2018 Jun 25. PubMed Article

Plasmids from Article

ID	Plasmid	Purpose
117754	pCarCBA	Codon optimized CarCBA enzymes from Pectobacterium carotovorum in pBbaA5k backbone
117755	pCarCBAE	Codon optimized CarCBAE enzymes from Pectobacterium carotovorum in pBbaA5k backbone
117756	pCarCBADE_ProB(I69E)A	ProB(I69E)A and codon optimized CarCBADE enzymes (in that order) in pBbaA5k backbone
117757	pCarCBADE	Codon optimized CarCBADE enzymes from Pectobacterium carotovorum in pBbaA5k backbone

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