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Capturing the Onset of PRC2-Mediated Repressive Domain Formation.

Oksuz O, Narendra V, Lee CH, Descostes N, LeRoy G, Raviram R, Blumenberg L, Karch K, Rocha PP, Garcia BA, Skok JA, Reinberg D
Mol Cell. 2018 Jun 21;70(6):1149-1162.e5. doi: 10.1016/j.molcel.2018.05.023. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
131321EED-KO-gRNA-1gRNA to knockout EED. Use with EED-KO-gRNA-2
131322EED-KO-gRNA-2gRNA to knockout EED. Use with EED-KO-gRNA-1
131323Y365A or A365Y inducible rescue gRNA to generate i-WT-r or i-MT-r systems
131324A365Y-GFP Donor for iWTr EEDDonor to generate i-WT-r system
131325Y365A-GFP Donor for iMTr EEDDonor to generate i-MT-r system
131326EED-F97AgRNA to generate F97A cage-mutant EED
131327JARID2-KO-gRNA-2 gRNA to knockout JARID2. Use with JARID2-KO-gRNA-1
131328JARID2-KO-gRNA-1 gRNA to knockout JARID2. Use with JARID2-KO-gRNA-2
131329EZH2-Promoter-gRNAgRNA to generate endogenously tagged -tetR-EZH2
131331SKOR1-gRNA-N-delgRNA to delete a nucleation site near Skor1. Use with SKOR1-gRNA-C-del
131332SKOR1-gRNA-C-delgRNA to delete a nucleation site near Skor1. Use with SKOR1-gRNA-N-del
131333LMX1B-gRNA-N-delgRNA to delete a nucleation site near Lmx1b. Use with LMX1B-gRNA-C-del
131334MTF2_KO_ex11_gRNA-4gRNA to knockout MTF2. This gRNA is also used in Haojie Li et al. Nature 2017. It targets exon 11 of MTF2.
131335HoxD del control usgRNA to delete control region near HoxD. Use with HoxD del control ds
131336HoxD del control dsgRNA to delete control region near HoxD. Use with HoxD del control us
131337HoxD del us1gRNA to delete a nucleation site near HoxD. Use with HoxD del ds1
131338HoxD del ds1gRNA to delete a nucleation site near HoxD. Use with HoxD del us1
1313395xtetO (tet3) Gene DesertgRNA to insert 5xTet operator sequence into a gene desert on chromosome 5.
1313405xtetO HoxC 3'gRNA to insert 5xTet operator sequence into downstream of HoxC cluster.
131341HoxC-downstream-5xTetO DonorDonor template to insert 5xTet operator sequence into downstream of HoxC cluster.
131516LMX1B-gRNA-C-delgRNA to delete a nucleation site near Lmx1b. Use with LMX1B-gRNA-N-del

Antibodies from Article