Encoding human serine phosphopeptides in bacteria for proteome-wide identification of phosphorylation-dependent interactions.

Barber KW, Muir P, Szeligowski RV, Rogulina S, Gerstein M, Sampson JR, Isaacs FJ, Rinehart J
Nat Biotechnol. 2018 Aug;36(7):638-644. doi: 10.1038/nbt.4150. Epub 2018 Jun 11. PubMed Article

Plasmids from Article

ID	Plasmid	Purpose
111704	Mode #1 Library	Encodes GST-fused human phosphopeptides
111705	Mode #2 Library (14-3-3β)	Encodes 14-3-3β and human phosphopeptides fused to split mCherry for Hi-P
111706	Mode #2 Library (14-3-3σ)	Encodes 14-3-3σ and human phosphopeptides fused to split mCherry for Hi-P
111707	Mode #2 Library (NEDD4 WW2 domain)	Encodes NEDD4 WW2 domain and human phosphopeptides fused to split mCherry for Hi-P
111708	Mode #2 Library (NEDD4-2 WW2 domain)	Encodes NEDD4-2 WW2 domain and human phosphopeptides fused to split mCherry for Hi-P
111880	pNAS1b split mCherry 14-3-3β	Mode #2 phosphosite expression (split mCherry, using 14-3-3β binding domain)
111881	pNAS1b split mCherry 14-3-3σ	Mode #2 phosphosite expression (split mCherry, using 14-3-3σ binding domain)
111882	pNAS1b split mCherry NEDD4 WW2	Mode #2 phosphosite expression (split mCherry, using NEDD4 WW2 binding domain)
111883	pNAS1b split mCherry NEDD4-2 WW2	Mode #2 phosphosite expression (split mCherry, using NEDD4-2 WW2 binding domain)
112029	pNAS1b split mCherry NEDD4 WW2 pos ctrl	Mode #2 phosphosite positive control (split mCherry, using NEDD4 WW2 binding domain paired with proline-rich positive control peptide)
112030	pNAS1b split mCherry NEDD4 WW2 neg ctrl	Mode #2 phosphosite negative control (split mCherry, using NEDD4 WW2 binding domain paired with negative control peptide)
112031	pNAS1b split mCherry 14-3-3β ctrl	Mode #2 phosphosite control (split mCherry, using 14-3-3β binding domain paired with phospho-dependent interacting peptide with TAG codon)