Semisynthetic biosensors for mapping cellular concentrations of nicotinamide adenine dinucleotides.
Sallin O, Reymond L, Gondrand C, Raith F, Koch B, Johnsson K
eLife. 2018 May 29;7. pii: 32638. doi: 10.7554/eLife.32638.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 113658 | pET-51b(+)_NAD-Snifit | Plasmid encoding the bacterial expression of the FRET semi-synthetic biosensor measuring NAD+ concentration |
| 113913 | pET-51b(+)_NADP-Snifit | Plasmid encoding the bacterial expression of the FRET semi-synthetic biosensor measuring the ratio NADPH/NADP+ |
| 113914 | pEBTet_NAD-Snifit-cyto | Plasmid encoding the cytosolic localization of the FRET semi-synthetic biosensor measuring NAD+ concentration in mammalian cells |
| 113915 | pEBTet_NAD-Snifit-nucl | Plasmid encoding the nuclear localization of the FRET semi-synthetic biosensor measuring NAD+ concentration in mammalian cells |
| 113916 | pEBTet_NAD-Snifit-mito | Plasmid encoding the mitochondrial localization of the FRET semi-synthetic biosensor measuring NAD+ concentration in mammalian cells |
| 113917 | pEBTet_NADP-Snifit-cyto | Plasmid encoding the cytosolic localization of the FRET semi-synthetic biosensor measuring the ratio NADPH/NADP+ in mammalian cells |
| 113918 | pEBTet_NADP-Snifit-nucl | Plasmid encoding the nuclear localization of the FRET semi-synthetic biosensor measuring the ratio NADPH/NADP+ in mammalian cells |
| 113919 | pEBTet_NADP-Snifit-mito | Plasmid encoding the mitochondrial localization of the FRET semi-synthetic biosensor measuring the ratio NADPH/NADP+ in mammalian cells |