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Addgene
An ingestible bacterial-electronic system to monitor gastrointestinal health.
Mimee M, Nadeau P, Hayward A, Carim S, Flanagan S, Jerger L, Collins J, McDonnell S, Swartwout R, Citorik RJ, Bulovic V, Langer R, Traverso G, Chandrakasan AP, Lu TK
Science. 2018 May 25;360(6391):915-918. doi: 10.1126/science.aas9315.
PubMed Article

Plasmids from Article

ID Plasmid Purpose
112529pMM532pZA1D-hrtR - HrtR expressed consitutively from promoter ProD with RBS2, p15a origin, AmpR
112530pMM534pZE2-PLhrtO-luxCDABE - Tandem LuxCDABE expressed constitutively from promoter PLhrtO, ColE1 origin, KanR
112531pMM549pZA1D-hrtR-chuA - HrtR expressed consitutively from promoter ProD with RBS2, ChuA expressed consitiutively from promoter J23107, p15a origin, AmpR
112532pMM627pZE2-PLhrtO-lux-hrtR-RBS2-chuA - Composite plasmid of pMM534 and pMM549, ColE1 origin, KanR
112533pMM643pZE2-PLhrtO-lux-hrtR-RBS3-chuA - HrtR RBS variant of plasmid pMM627 (Strength 33545.5 AU), ColE1 origin, KanR
112534pMM1157pZE2-PLhrtO-lux-hrtR-RBS3 - ChuA transciptional unit deletion of plasmid pMM643, ColE1 origin, KanR
112535pMM1162pZE2-hrtR-RBS3-chuA - LuxCDABE transcriptional unit deletion of plasmid pMM643, ColE1 origin, KanR

Antibodies from Article