Transfection of Capsaspora owczarzaki, a close unicellular relative of animals.
Parra-Acero H, Ros-Rocher N, Perez-Posada A, Kozyczkowska A, Sanchez-Pons N, Nakata A, Suga H, Najle SR, Ruiz-Trillo I
Development. 2018 May 11. pii: dev.162107. doi: 10.1242/dev.162107. PubMed Article
Parra-Acero H, Ros-Rocher N, Perez-Posada A, Kozyczkowska A, Sanchez-Pons N, Nakata A, Suga H, Najle SR, Ruiz-Trillo I
Development. 2018 May 11. pii: dev.162107. doi: 10.1242/dev.162107. PubMed Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 111873 | pONSY | "Capsaspora owczarzaki" expression vector (backbone) modified from the pCR2.1. It bears the promoter and terminator regions from the endogenous Elongation Factor 1 alpha (EF1a) gene (CAOG_07807). |
| 111874 | pONSY-mCherry | This plasmid expresses mCherry fluorescent protein under endogenous EF1a promoter. It can be used to transfect "Capsaspora owczarzaki" cells and visualize them by fluorescence microscopy. |
| 111875 | pONSY-Venus | This plasmid expresses Venus fluorescent protein under endogenous EF1a promoter. It can be used to transfect "Capsaspora owczarzaki" cells and visualize them by fluorescence microscopy. |
| 111876 | pONSY-Lifeact:mCherry | This plasmid expresses mCherry fused to Lifeact peptide. It labels actin bundles and filopodia in Capsaspora. Codons have been optimised according to their usages in Capsaspora and C. fragrantissima. |
| 111877 | pONSY-CoH2B:Venus | This plasmid expresses Venus fluorescent protein fused to endogenous Histone H2B (H2B) gene of Capsaspora (CAOG_01818). It can be used to transfect Capsaspora cells and visualize nucleus in vivo. |
| 111878 | pONSY-CoNMM:mCherry | This plasmid expresses mCherry fluorescent protein fused to an N-Myristoylation motif (NMM) from the endogenous Src2 gene (CAOG_06360). It can be used to visualize the membrane and filopodia. |