C-BERST: defining subnuclear proteomic landscapes at genomic elements with dCas9-APEX2.

Gao XD, Tu LC, Mir A, Rodriguez T, Ding Y, Leszyk J, Dekker J, Shaffer SA, Zhu LJ, Wolfe SA, Sontheimer EJ
Nat Methods. 2018 Jun;15(6):433-436. doi: 10.1038/s41592-018-0006-2. Epub 2018 May 7. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID	Plasmid	Purpose
108570	pEJS578_DD-dSpyCas9-mCherry-APEX2	Expression of drug-inducible dSpyCas9-mCherry-APEX2 for biotin-labeling genomic element-associated protein factors
108649	pEJS613_pTetR-P2A-BFPnls/sgTelo	Telomere-targeting Spy sgRNA under U6 promoter
108650	pEJS614_pTetR-P2A-BFPnls/sgNS	Non-specific Spy sgRNA under U6 promoter
108651	pEJS887_pTetR-P2A-BFPnls/sgAlpha	Alpha satellite repeats targeting Spy sgRNA under U6 promoter