New CRISPR Mutagenesis Strategies Reveal Variation in Repair Mechanisms among Fungi.
Vyas VK, Bushkin GG, Bernstein DA, Getz MA, Sewastianik M, Barrasa MI, Bartel DP, Fink GR
mSphere. 2018 Apr 25;3(2). pii: 3/2/e00154-18. doi: 10.1128/mSphere.00154-18. Print 2018 Apr 25.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 111430 | pV1393 | CaCas9/gRNA Solo entry plasmid for cloning guides - contains stuffer with BsmBI sites - inserts at Neut5L - Recyclable for serial mutagenesis (Sap2-FLP) |
| 111431 | pV1524 | CaCas9/gRNA Solo entry plasmid for cloning guides - contains stuffer with BsmBI sites - inserts at Neut5L - Recyclable for serial mutagenesis (Mal2-FLP) |
| 111434 | pV1539 | Genedrive entry vector for Candida albicans |
| 111435 | pV1326 | S. cerevisiae and C. glabrata Solo CRISPR vector, marked with URA3 and NatR |
| 111436 | pV1382 | S. cerevisiae and C. glabrata Solo CRISPR vector, marked with URA3 and NatR |
| 111438 | pV1329 | Solo vector pV1326 +sgCgADE2 |
| 111439 | pV1435 | Solo vector pV1382 + sgCgADE2 |
| 111440 | pV1464 | N. castellii Solo CRISPR vector, marked with URA3 and NatR |
| 111443 | pV1338 | Solo vector pV1326 + sgScADE2 |
| 111444 | pVG1 | Unified Solo vector pV1382 + sgScADE2 + ScADE2 stop codon repair tempate |
| 111445 | pV1386 | Solo vector pV1382 + sgScADE2 |