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Genome-wide determination of on-target and off-target characteristics for RNA-guided DNA methylation by dCas9 methyltransferases.
Lin L, Liu Y, Xu F, Huang J, Daugaard TF, Petersen TS, Hansen B, Ye L, Zhou Q, Fang F, Yang L, Li S, Floe L, Jensen KT, Shrock E, Chen F, Yang H, Wang J, Liu X, Xu X, Bolund L, Nielsen AL, Luo Y
Gigascience. 2018 Mar 1;7(3):1-19. doi: 10.1093/gigascience/giy011.
PubMed Article

Plasmids from Article

ID Plasmid Purpose  
66818pCCL-PGK-SPdCas9-BFP-DNMT1dCas9 fused to BFP and the human DNMT1 catalytic domain
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66819pCCL-PGK-SPdCas9-BFP-DNMT3AdCas9 fused to BFP and the human DNMT3A catalytic domain
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66820pCCL-PGK-SPdCas9-BFP-DNMT3BdCas9 fused to BFP and the human DNMT3B catalytic domain
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66821pCCL-PGK-SPdCas9-BFP-EGFPdCas9 fused to BFP and EGFP
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71213pCCL-PGK-SPdCas9-BFP-DNMT3A(E752A)dCas9 fused to BFP and the human DNMT3A catalytically inactive domain
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71214pCCL-PGK-SPdCas9-BFP-DNMT3B(E697A)dCas9 fused to BFP and the human DNMT3B catalytically inactive domain
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71215pLenti-EF1a-SPdCas9-EGFP-2A-BlastdCas9 fused to EGFP
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71216pLenti-EF1a-SPdCas9-DNMT3A-2A-BlastdCas9 fused to human DNMT3A catalytic domain
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71217pLenti-EF1a-SPdCas9-DNMT3B-2A-BlastdCas9 fused to human DNMT3B catalytic domain
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71218pLenti-EF1a-SPdCas9-DNMT3A(E752A)-2A-BlastdCas9 fused to the human DNMT3A catalytically inactive domain
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71219pLenti-EF1a-SPdCas9-DNMT3B(E697A)-2A-BlastdCas9 fused to the human DNMT3B catalytically inactive domain
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Antibodies from Article