Genetic Engineering of Bee Gut Microbiome Bacteria with a Toolkit for Modular Assembly of Broad-Host-Range Plasmids.
Leonard SP, Perutka J, Powell JE, Geng P, Richhart DD, Byrom M, Kar S, Davies BW, Ellington AD, Moran NA, Barrick JE
ACS Synth Biol. 2018 May 18;7(5):1279-1290. doi: 10.1021/acssynbio.7b00399. Epub 2018 Apr 13. PubMed Article
Leonard SP, Perutka J, Powell JE, Geng P, Richhart DD, Byrom M, Kar S, Davies BW, Ellington AD, Moran NA, Barrick JE
ACS Synth Biol. 2018 May 18;7(5):1279-1290. doi: 10.1021/acssynbio.7b00399. Epub 2018 Apr 13. PubMed Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 110573 | pBTK001 | Broad-host-range toolkit (BTK) entry vector, with superfolder GFP |
| 110574 | pBTK102 | BTK Type 2 promoter for golden gate assembly |
| 110575 | pBTK107 | BTK Type 2 promoter for golden gate assembly |
| 110576 | pBTK110 | BTK Type 2 promoter for golden gate assembly |
| 110577 | pBTK112 | BTK Type 2 promoter for golden gate assembly |
| 110578 | pBTK113 | BTK Type 2 promoter for golden gate assembly |
| 110581 | pBTK121 | BTK Type 2 promoter for golden gate assembly |
| 110582 | pBTK103 | BTK Type 2 promoter for golden gate assembly |
| 110583 | pBTK124 | BTK Type 2 promoter for golden gate assembly |
| 110584 | pBTK130 | BTK Type 2 promoter for golden gate assembly |
| 110587 | pBTK205 | BTK Type 3 coding sequence for golden gate assembly |
| 110588 | pBTK206 | BTK Type 3 coding sequence for golden gate assembly |
| 110590 | pBTK224 | BTK Type 3 coding sequence for golden gate assembly |
| 110591 | pBTK229 | BTK Type 3 coding sequence for golden gate assembly and homologous recombination |
| 110592 | pBTK300 | BTK Type 4 terminator for golden gate assembly |
| 110593 | pBTK301 | BTK Type 6-7 terminator (replaces Type 6 and Type 7 parts) |
| 110594 | pBTK305 | BTK Type 4 terminator for golden gate assembly |
| 110595 | pBTK211 | BTK Type 6 Repressor CDS for golden gate assembly |
| 110597 | pBTK401 | BTK Type 8 Bacterial origin (broad-host-range) for golden gate assembly |
| 110598 | pBTK402 | BTK Type 8 Bacterial origin (broad-host-range) for golden gate assembly |
| 110599 | pBTK403 | BTK Type 8 Bacterial origin (broad-host-range) for golden gate assembly |
| 110600 | pBTK599 | BTK Type 8 Bacterial origin (R6K suicide plasmid) for homologous recombination |
| 110601 | pBTK599s | BTK Type 8 Bacterial origin (R6K suicide plasmid) for homologous recombination |
| 110602 | pBTK501 | BTK assembled plasmid- broad-host-range GFP expression from PA1 promoter (ampicillin resistance) |
| 110603 | pBTK519 | BTK assembled plasmid- broad-host-range GFP expression from PA1 promoter (kanamycin resistance) |
| 110604 | pBTK520 | BTK assembled plasmid- broad-host-range GFP expression from PA1 promoter (spectinomycin resistance) |
| 110605 | pBTK527 | BTK Assembled plasmid- ready for Stage 2 assembly (ConLS' and ConRE' connectors with kanamycin resistance) |
| 110606 | pBTK527a | BTK Assembled plasmid- ready for Stage 2 assembly (ConLS' and ConRE' connectors with spectinomycin resistance) |
| 110607 | pBTK601 | BTK assembled plasmid - encodes Cas9 with no targeting sgRNA (used with suicide plasmid for broad-host-range genome alteration) |
| 110608 | pBTK614 | BTK assembled plasmid - Encodes dCas9 with no targeting sgRNA (used in Stage 2 assembly to repress target genes) |
| 110609 | pBTK615 | BTK assembled plasmid - used in Stage 2 assembly to target Cas9 or dCas9 |
| 110610 | pBTK509 | BTK assembled broad-host-range plasmid encoding GFP driven by PA2 promoter |
| 110611 | pBTK510 | BTK assembled broad-host-range plasmid encoding GFP driven by PA3 promoter |
| 110612 | pBTK563 | BTK assembled broad-host-range plasmid encoding NanoLuc luciferase protein driven by CP25 promoter |
| 110613 | pBTK564 | BTK assembled broad-host-range plasmid encoding NanoLuc luciferase protein driven by PA1 promoter |
| 110614 | pBTK569 | BTK assembled broad-host-range plasmid encoding E2-Crimson protein driven by CP25 promoter |
| 110615 | pBTK570 | BTK assembled broad-host-range plasmid encoding E2-Crimson protein driven by PA3 promoter |
| 110616 | pBTK503 | BTK assembled broad-host-range plasmid encoding GFP driven by CP25 promoter |
| 110617 | pBTK550d | BTK assembled broad-host-range plasmid encoding inducible GFP driven by T7 polymerase |
| 110618 | pBTK552 | BTK assembled broad-host-range plasmid encoding inducible GFP driven CP25 promoter with lac operator. Also encodes LacI. |
| 110619 | pBTK619 | BTK assembled broad-host-range plasmid encoding GFP driven by CP12b promoter |
| 110620 | pBTK620 | BTK assembled broad-host-range plasmid encoding GFP driven by CP32 promoter |
| 110621 | pBTK621 | BTK assembled broad-host-range plasmid encoding GFP driven by CP6 promoter |